NIR-Remote Selected Activation Gene Expression in Living Cells by Upconverting Microrods
NIR-Remote Selected Activation Gene Expression in Living Cells by Upconverting Microrods
复制标题
通过上转换微棒近红外远程选择活细胞中的激活基因表达
DOI：
10.1002/adma.201503961
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发表时间：
2016
影响因子：
29.4
通讯作者：
Chang Jin
中科院分区：
文献类型：
--
作者：
Zheng Bin;Su Lin;Pan Huizhuo;Hou Beibei;Zhang Ying;Zhou Fang;Wu Xiaoli;Gong Xiaoqun;Wang Hanjie;Chang Jin
DOI：10.1002/adma。 201503961 解笼过程中的囊泡。在这些囊泡中，二氧化硅纳米粒子由于表面积大、孔径容易改变、负载体积大以及能够进行表面化学修饰，因此对于药物递送应用非常有吸引力。本文成功设计并制备了用于近红外光触发基因释放系统的与mSiO 2@ PEI纳米载体共轭的上转换棒的构建方案。这种新的基因纳米载体系统可以用作生物技术和基于基因的药物设计中精确调节基因表达的新技术。作为我们研究的起点，基因纳米载体的制备如图1A所示。通过 FTIR 光谱确定基因纳米载体的化学结构。 FTIR光谱显示，mSiO 2 纳米颗粒表面经3-氨丙基三乙氧基硅烷(APTES)修饰后，在对应于C− N振动的1350 cm−1处和对应于N− H振动的1580 cm−1处出现了新峰，表明氨基已成功共轭到SiO 2 表面。mSiO 2-NH 2 纳米颗粒经马来酸酐修饰后，新的强峰出现在对应于N− H振动的1580 cm− 1处。在 1670 cm−1 处观察到峰值，对应于羧基的 C= O 振动。然后将PEI与mSiO 2-COOH纳米粒子缀合，并引入大量氨基，氨基峰在1580 cm−1处增加，表明PEI分子已成功包覆在mSiO 2-COOH表面（图1 B）。通过 TEM 表征了纳米颗粒的形貌。如图 1 C 所示，mSiO 2 纳米颗粒均匀且单分散，尺寸为 90-100 nm。 TEM图像表明，mSiO_2-COOH纳米粒子表面修饰PEI后，纳米粒子的形貌仍然均匀且单分散。图1D显示mSiO 2@PEI纳米载体的尺寸约为100-110 nm，略大于mSiO 2 纳米颗粒的尺寸。此外，纳米颗粒含有许多纳米孔（图1C和1D），也可用于加载染料以追踪基因纳米载体（孔半径测量结果如图S1，支持信息所示）。所有样品的粒径均通过 Zeta PALS (BIC) 进行测试（图 1 E 和图 S2，支持信息）。图1F和S3显示mSiO 2@ PEI纳米载体的Zeta电位为54.48 mV，表明mSiO 2@ PEI纳米载体可能是比mSiO 2-NH 2 纳米粒子更好的凝聚DNA的载体（图S4，支持信息）。与游离PEI（分子量1800）相比，负载DNA的mSiO 2@ PEI纳米载体表现出更高的结合效率（图S5，支持信息）和基因转染
DOI: 10.1002/adma. 201503961 by the vesicles during the uncaging process. Among these vesicles, silica nanoparticles are very attractive for drug delivery applications due to the large surface area, easily modified pore size, large volume for cargo loading and the ability to undergo surface chemical modification. In this paper, a protocol for the construction of upconversion rods conjugated with a mSiO 2@ PEI nanocarrier for a NIR light-triggered gene release system has been designed and prepared successfully. This new gene nanocarrier system can be used as a novel technique to precisely regulate gene expression in biotechnology and genebased medicine design.For a starting point of our study, the gene nanocarriers were prepared as shown in Figure 1A. The chemical structure of the gene nanocarriers was determined by FTIR spectra. The FTIR spectra show that after decoration of the mSiO 2 nanoparticles with 3-aminopropyltriethoxysilane (APTES) on the surface, new peaks appeared at 1350 cm− 1 corresponding to a C− N vibration and 1580 cm− 1 corresponding to a N− H vibration, which indicated that the amino groups had been successfully conjugated onto the surface of SiO 2. After the mSiO 2-NH 2 nanoparticles were modified by maleic anhydride, a new intensive peak was observed at 1670 cm− 1 corresponding to a C= O vibration of carboxyl groups. Then the PEI was conjugated with mSiO 2-COOH nanoparticles and a mass of amino groups were introduced, and the peaks of the amino groups increased at 1580 cm− 1, indicating that the PEI molecules had coated the surface of the mSiO 2-COOH successfully (Figure 1 B). The morphologies of the nanoparticles were characterized by TEM. As shown in Figure 1 C, the mSiO 2 nanoparticles were uniform and monodisperse with sizes of 90–100 nm. The TEM images indicate that the morphology of the nanoparticles was still uniform and monodisperse after PEI was decorated on the surface of the mSiO 2-COOH nanoparticles. Figure 1 D revealed that the size of mSiO 2@ PEI nanocarriers was about 100–110 nm, which was slightly larger than that of the mSiO 2 nanoparticles. Besides, the nanoparticles contained many nanopores (Figure 1 C and 1D), which can also be used for loading dyes to track the gene nanocarriers (pore radius measurements are shown in Figure S1, Supporting Information). The particle size of all samples was tested by the Zeta PALS (BIC)(Figure 1 E and Figure S2, Supporting Information). Figure 1 F and S3 show that the Zeta potential of mSiO 2@ PEI nanocarriers was 54.48 mV, showing that mSiO 2@ PEI nanocarriers may be better carriers to condense DNA than mSiO 2-NH 2 nanoparticles (Figure S4, Supporting Information). Compared with free PEI (Mw. 1800), the DNA-loaded mSiO 2@ PEI nanocarrier showed a much higher combination efficiency (Figure S5, Supporting Information) and gene transfection