Lysozyme Solubility and Conformation in Neat Ionic Liquids and Their Mixtures with Water.
Lysozyme Solubility and Conformation in Neat Ionic Liquids and Their Mixtures with Water.
复制标题
溶菌酶在纯离子液体及其与水的混合物中的溶解度和构象。
DOI：
10.1021/acs.biomac.6b00468
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发表时间：
2016
影响因子：
6.2
通讯作者：
D. Hoagland
中科院分区：
文献类型：
--
作者：
Stephen H. Strassburg;H. Bermudez;D. Hoagland
一些模型蛋白质的室温溶解度进行了评估，为不同的净离子液体（IL）的集合。对于两种可溶性蛋白-IL对，[C2MIM][EtSO 4]中的溶菌酶（1-乙基-3-甲基咪唑鎓乙基硫酸盐）和[C2，4，4，4P][Et 2 PO 4]中（三丁基（乙基）磷二乙基磷酸盐），蛋白质的溶解性和结构在不同温度下探测动态光散射（评估溶解的分子大小），比浊法（反映溶解度）和傅里叶变换红外光谱（揭示螺旋二级结构）。与水性环境相比，[C2，4，4，4P][Et 2 PO 4]热稳定蛋白质大小和二级结构，而[C2MIM][EtSO 4]则相反。在[C2MIM][EtSO 4]中变性的溶菌酶不会聚集，可能是由于不存在疏水相互作用，并且变性似乎是热可逆的。两种IL在室温下都可以以所有比例与水混溶，但为了与蛋白质形成相应的三元混合物，混合顺序很重要。混合以避免向IL-溶解的蛋白质中加入水，在所有溶剂组成下用[C2MIM][EtSO 4]获得稳定的溶液。当将水加入到富含IL的溶液中时，注意到液-液分层。
The room temperature solubility of a number of model proteins is assessed for a diverse set of neat ionic liquids (ILs). For two soluble protein-IL pairs, lysozyme in [C2MIM][EtSO4] (1-ethyl-3-methylimidazolium ethylsulfate) and in [C2,4,4,4P][Et2PO4] (tributyl(ethyl)phosphonium diethylphosphate), protein solubility and structure at various temperatures are probed by dynamic light scattering (assessing dissolved molecular size), turbidimetry (reflecting degree of solubility), and Fourier transform infrared spectroscopy (uncovering helical secondary structure). As compared to aqueous environments, [C2,4,4,4P][Et2PO4] thermally stabilizes protein size and secondary structure while [C2MIM][EtSO4] does the opposite. Lysozyme denatured in [C2MIM][EtSO4] does not aggregate, presumably due to an absence of hydrophobic interactions, and the denaturation appears thermally reversible. Both ILs at room temperature are miscible with water in all proportions, but to create the corresponding ternary mixtures with protein, the order of mixing is important. Mixed to avoid additions of water to IL-dissolved protein, stable solutions are obtained with [C2MIM][EtSO4] at all solvent compositions. When water is added to IL-rich solutions, liquid-liquid demixing is noted.
DOI：
10.1039/c1cp22965f
发表时间：
2012-01
期刊：
Physical chemistry chemical physics : PCCP
影响因子：
--
作者：
K. D. Weaver;Regina M. Vrikkis;Matthew P Van Vorst;Jonathan Trullinger;R. Vijayaraghavan;D. Foureau;I. McKillop;D. Macfarlane;J. Krueger;G. Elliott
通讯作者：
K. D. Weaver;Regina M. Vrikkis;Matthew P Van Vorst;Jonathan Trullinger;R. Vijayaraghavan;D. Foureau;I. McKillop;D. Macfarlane;J. Krueger;G. Elliott
影响因子：
6.2
作者：
Wei, Wenjun;Danielson, Neil D.
通讯作者：
Danielson, Neil D.
影响因子：
3.4
作者：
Baldwin, RL
通讯作者：
Baldwin, RL