CHANGES IN MAJOR PHOSPHOLIPIDS OF MITOCHONDRIA DURING POSTISCHEMIC REPERFUSION IN RAT-BRAIN
CHANGES IN MAJOR PHOSPHOLIPIDS OF MITOCHONDRIA DURING POSTISCHEMIC REPERFUSION IN RAT-BRAIN
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DOI：
10.3171/jns.1992.76.2.0244
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发表时间：
1992-02-01
影响因子：
4.1
通讯作者：
OGATA, N
中科院分区：
文献类型：
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作者：
NAKAHARA, I;KIKUCHI, H;OGATA, N
主要的线粒体磷脂在大鼠脑缺血30分钟或60分钟后，再灌注30分钟后进行了检查，以检查它们的变化，并探讨它们与缺血后再灌注期间线粒体功能障碍的关系。 磷脂的量和多不饱和脂肪酸链的百分比，在缺血30分钟期间趋于减少，在再灌注后恢复。 然而，缺血持续60分钟后，这些参数没有恢复，但进一步下降，表明再灌注后磷脂酶A2的磷脂进行性破坏。 这些变化在心磷脂中尤其显着，心磷脂专门存在于线粒体中。 这些变化还与线粒体呼吸和呼吸酶（细胞色素c氧化酶和F0 F1-腺苷三磷酸酶）活性密切相关，已知这些酶与心磷脂的量相关。 这些结果表明，线粒体膜磷脂代谢是一个重要因素，在缺血后再灌注的能量代谢的完整性。
Major mitochondrial phosopholipids were examined in rat brain after 30 minutes of reperfusion following 30- or 60-minute periods of ischemia to examine their changes and explore their relationship to mitochondrial dysfunction during postischemic reperfusion. The amount of phospholipids and the percentage of polyunsaturated fatty acid chains, which tended to decrease during 30 minutes of ischemia, recovered after reperfusion. However, after ischemia lasting for 60 minutes, these parameters did not recover but decreased further, suggesting progressive disruption of phospholipids by phospholipase A2 after reperfusion. These changes were particularly notable in cardiolipin, which is contained specifically in mitochondria. The changes were also closely associated with mitochondrial respiration and respiratory enzyme (cytochrome c oxidase and F0F1-adenosine triphosphatase) activities, which have been known to correlate with the amount of cardiolipin. These results suggest that phospholipid metabolism in mitochondrial membranes is an important factor bearing on the integrity of energy metabolism during postischemic reperfusion.