Perfluorocarbon Nanocapsules Improve Hypoxic Microenvironment for the Tumor Ultrasound Diagnosis and Photodynamic Therapy
Perfluorocarbon Nanocapsules Improve Hypoxic Microenvironment for the Tumor Ultrasound Diagnosis and Photodynamic Therapy
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DOI：
10.1166/jbn.2018.2656
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发表时间：
2018-12-01
影响因子：
2.9
通讯作者：
He, Dannong
中科院分区：
文献类型：
--
作者：
Li, Niannian;Xu, Fei;He, Dannong
改善缺氧是提高抗肿瘤治疗效果的重要因素。在本实验中，已经开发了聚（丙交酯-共-乙交酯）-聚（乙二醇）/全氟辛基溴（PFOB）/光敏剂（IR 780）的纳米胶囊，以增加肿瘤内的氧浓度并改善光动力治疗（PDT）的结果。PFOB具有高的氧溶解度，IR 780是特别适合于在808-nm激光照射下的高效光动力疗法的光敏剂。因此，PFOB为与光敏剂的反应提供足够的氧，产生更多的单线态氧以诱导细胞凋亡。通过缺氧微环境的体外模拟实验，证实加入PFOB后纳米胶囊的光动力学效应增强。结果表明，由于添加了PFOB，纳米胶囊可以提供对比增强的超声诊断，并确保在808 nm激光照射后对肺肿瘤生长的更显著抑制。此外，纳米胶囊允许光敏剂实现足够的治疗潜力，这在约5%氧浓度的适度缺氧条件下是最佳的。
Amelioration of hypoxia is an important factor increasing the effects of anti-tumor therapies. In the present experiments, nanocapsules of poly(lactide-co-glycolide)-poly(ethylene glycol)/perfluorooctyl bromide (PFOB)/photosensitizers (IR780) have been developed to increase oxygen concentration inside the tumor and improve the outcome of photodynamic therapy (PDT). PFOB has high oxygen solubility, and IR780 is a photosensitizer particularly suited for a highly effective photodynamic therapy under the 808-nm laser irradiation. Thus, PFOB provides sufficient oxygen for the reaction with the photosensitizer, producing more singlet oxygen to induce cell apoptosis. The photodynamic effect of nanocapsules was confirmed to be enhanced after addition of PFOB through simulation experiments in vitro in the hypoxic microenvironment. The results have demonstrated that due to the addition of PFOB the nanocapsules can provide a contrast-enhanced ultrasound diagnosis and ensure more notable inhibition of the growth of lung tumors following the 808 nm laser irradiation. Furthermore, the nanocapsules allowed the photosensitizers to achieve sufficient therapeutic potential, which was optimal under the conditions of modest hypoxia with about 5% oxygen concentration.