A Synechocystis PCC 6803 psbA Deletion Mutant and Its Transformation with a psbA Gene from a Higher Plant
A Synechocystis PCC 6803 psbA Deletion Mutant and Its Transformation with a psbA Gene from a Higher Plant
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集胞藻 PCC 6803 psbA 缺失突变体及其高等植物 psbA 基因的转化
DOI：
10.1007/978-94-009-0511-5_106
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
B. Diner
中科院分区：
文献类型：
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作者：
P. Nixon;J. Metz;M. Roegner;B. Diner
单细胞蓝细菌synechocystispcc 6803被证明是研究类囊体膜蛋白复合物，特别是光系统2 （PS2）的有价值的模式生物(1)。它在葡萄糖存在下的光异养生长能力，以及它的天然DNA转化系统，允许通过各种方法产生PS2功能缺陷的突变体，并研究了它们的性质(2)。我们的团队对通过位点导向突变体的生化和生物物理特征来理解Dl多肽的功能感兴趣。这种方法由于每条染色体上存在三个不同的psba基因拷贝而受到阻碍(3)。作为产生突变体的第一步，我们在这里描述了apsbA缺失突变体的构建和初步表征。此外，我们已经能够通过转化来自高等植物poa annua的psba基因来恢复该缺失菌株的光自养生长。
The unicellular cyanobacteriumSynechocystisPCC 6803 is proving to be a valuable model organism for the study of thylakoid-membrane protein complexes, particularly Photosystem Two (PS2) (1). Its ability to grow photoheterotrophically in the presence of glucose, together with its natural DNA transformation system, allow mutants defective in PS2 function to be generated by a variety of methods and their properties studied (2). Our group is interested in understanding the function of the Dl polypeptide through the biochemical and biophysical characterisation of site-directed mutants. Such an approach is hampered by the presence of three different copies of thepsbA gene for each chromosome (3). As a first step towards generating mutants, we describe here the construction and preliminary characterisation of apsbA deletion mutant. Futhermore, we have been able to restore photoautotrophic growth to this deletion strain by transformation with thepsbA gene from the higher plantPoa annua.
计算机物理通信。
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