Role of C-type natriuretic peptide in the function of normal human sperm.
Role of C-type natriuretic peptide in the function of normal human sperm.
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DOI：
10.4103/1008-682x.150254
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发表时间：
2016-01
影响因子：
2.9
通讯作者：
Huang DH
中科院分区：
文献类型：
--
作者：
Xia H;Chen Y;Wu KJ;Zhao H;Xiong CL;Huang DH
c型利钠肽（CNP）是一种新发现的局部调控因子，它通过特异性的膜结合的利钠肽受体-b （NPR-B）介导其生物学作用。近年来的研究表明，CNP与男性生殖功能密切相关。本研究的目的是通过不同的方法（如免疫定位、实时聚合酶链反应和Western Blot）确定CNP/NPR-B在人射精精子中的分布，然后评估CNP对体外精子功能（如运动能力和顶体反应）的影响。人类精液样本是从符合世界卫生组织正常精子症标准的自愿捐献者那里收集的。我们的研究结果表明，CNP的特异性受体NPR-B定位于精子头部顶体区域和尾部前端的膜，在人类精子中没有CNP的信号。与对照组相比，CNP可诱导精子活力和顶体反应显著呈剂量依赖性增加。综上所述，CNP/NPR-B可以影响精子活力和顶体反应，从而调节雄性生殖功能。CNP可能是调节精子功能的一个新的关键因子。
C-type natriuretic peptide (CNP) is a newly discovered type of local regulatory factor that mediates its biological effects through the specific, membrane-bound natriuretic peptide receptor-B (NPR-B). Recent studies have established that CNP is closely related to male reproductive function. The aims of this study were to determine the distribution of CNP/NPR-B in human ejaculated spermatozoa through different methods (such as immunolocalization, real time polymerase chain reaction and Western Blot), and then to evaluate the influence of CNP on sperm function in vitro, such as motility and acrosome reaction. Human semen samples were collected from consenting donors who met the criteria of the World Health Organization for normozoospermia. Our results show that the specific receptor NPR-B of CNP is localized in the acrosomal region of the head and the membrane of the front-end tail of the sperm, and there is no signal of CNP in human sperm. Compared with the control, CNP can induce a significant dose-dependent increase in spermatozoa motility and acrosome reaction. In summary, CNP/NPR-B can affect sperm motility and acrosome reaction, thus regulating the reproductive function of males. CNP may be a new key factor in regulating sperm function.