Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides
Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides
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DOI：
10.1093/humrep/den409
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发表时间：
2009-05-01
影响因子：
6.1
通讯作者：
Eisenbach, Michael
中科院分区：
文献类型：
--
作者：
Gakamsky, Anna;Armon, Leah;Eisenbach, Michael
哺乳动物精子趋化性中的一个主要问题是细胞是否通过比较时间点之间或空间点之间的化学引诱物浓度来感知化学引诱物梯度。我们在没有空间梯度的条件下，将人类精子暴露于时间化学引诱物梯度，通过将细胞与显微镜载玻片上的孕酮或Bourgeonal快速混合，并用运动分析软件分析它们的游泳。细胞在数秒内作出反应，速度和头部横向位移增加，游泳的线性下降，在反应的峰值处变得过度活化。所有的反应都是短暂的，持续了几秒钟。在细胞内光释放环磷酸腺苷或环磷酸鸟苷后，获得了基本上相似的结果，这些结果表明，人类精子的感觉和响应时间趋化梯度。在这些观察的基础上，我们提出了一个潜在的模型在空间趋化梯度的精子的趋化反应。
A major question in mammalian sperm chemotaxis is whether the cells sense a chemoattractant gradient by comparing the chemoattractant concentration between time points or between spatial points.To resolve this question, we exposed human spermatozoa to a temporal chemoattractant gradient under conditions of no spatial gradient by rapidly mixing the cells with progesterone or bourgeonal on a microscope slide and analyzing their swimming with motion analysis software.The cells responded within seconds with an increase in velocity and lateral head displacement, and with a decrease in the linearity of swimming, becoming hyperactivated at the peak of the response. All the responses were transient, lasting for a number of seconds. Essentially similar results were obtained upon intracellular photorelease of cyclic adenosine monophosphate or cyclic guanosine monophosphate, which are thought to be involved in mediating the chemotactic response.These results suggest that human spermatozoa sense and respond to a temporal chemoattractant gradient. On the basis of these observations, we propose a potential model for the chemotactic response of spermatozoa in a spatial chemoattractant gradient.