ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188
ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188
复制标题
ZmMPK6 和乙烯信号传导负向调节玉米自交系 A188 中抗昆虫代谢物 DIMBOA 和 DIMBOA-Glc 的积累。
DOI：
10.1111/nph.16974
复制
发表时间：
2020
期刊：
影响因子：
9.4
通讯作者：
Wu Jianqiang
中科院分区：
文献类型：
--
作者：
Zhang Cuiping;Li Jing;Li Sen;Ma Canrong;Liu Hui;Wang Lei;Qi Jinfeng;Wu Jianqiang
2，4-二羟基-7-甲氧基-1，4-苯并恶嗪-3-酮（丁布阿）和丁布阿葡糖苷本研究采用反向遗传学、激酶活性分析、植物激素和DIMBOA/DIMBOA-Glc定量、生物测定和转录组分析等方法，对ZmMPK 6（一种丝裂原活化蛋白激酶）的功能进行了研究，ZmMPK 6在玉米对植食的反应中，通过创伤和模拟植食被迅速激活。在玉米A188中沉默ZmMPK 6会损害模拟的植食性诱导的乙烯水平，但不会损害茉莉酸或水杨酸的乙烯水平，并且ZmMPK 6沉默的植物表现出升高的DIMBOA/DIMBOA-Glc和昆虫抗性。乙烯互补实验表明，ZmMPK 6以乙烯依赖性方式抑制DIMBOA/DIMBOA-Glc的积累。转录组分析表明，ZmMPK 6可能通过控制乙烯信号通路中的MYB转录因子来调控BX 1的转录，而且ZmMPK 6和乙烯信号通路还特异性地共同调控其他苯并恶嗪类生物合成基因的转录。这些结果揭示了ZmMPK 6和乙烯途径是DIMBOA/DIMBOA-Glc的新型阻遏物，并为这两条途径的调控机制提供了新的见解。
2,4‐Dihydroxy‐7‐methoxy‐1,4‐benzoxazin‐3‐one (DIMBOA) and DIMBOA‐glucoside (DIMBOA‐Glc) are anti‐insect benzoxazinoids in maize, yet very little information is known about how they are regulated.Reverse genetics, kinase activity analysis, phytohormone and DIMBOA/DIMBOA‐Glc quantification, bioassays and transcriptome analysis were employed to study the function of ZmMPK6, a mitogen‐activated protein kinase, in maize response to herbivory.ZmMPK6 was rapidly activated by wounding and simulated herbivory. SilencingZmMPK6in maize A188 compromised simulated herbivory‐induced ethylene levels but not those of jasmonic acid or salicylic acid, and theZmMPK6‐silenced plants exhibited elevated DIMBOA/DIMBOA‐Glc and insect resistance. An ethylene complementation experiment revealed that ZmMPK6 repressed the accumulation of DIMBOA/DIMBOA‐Glc in an ethylene‐dependent manner. Transcriptome analysis revealed that ZmMPK6 might meditate the transcription ofBX1by controlling a MYB transcription factor that is likely to be located in the ethylene signalling pathway and, furthermore, ZmMPK6 and ethylene signalling also specifically and commonly regulate the transcription of other benzoxazinoid biosynthetic genes. We also show that different maize lines have very different responses to simulated herbivory in terms of ZmMPK6 activation, ethylene emission and benzoxazinoid levels.These results uncover that ZmMPK6 and ethylene pathway are novel repressors of DIMBOA/DIMBOA‐Glc and provide new insight into the regulatory mechanisms underlying these two pathways.