Epigallocatechin-3-gallate reduces damage to osteoblast-like cells treated with Zoledronic acid
Epigallocatechin-3-gallate reduces damage to osteoblast-like cells treated with Zoledronic acid
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DOI：
10.1016/j.archoralbio.2018.06.018
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发表时间：
2018-10-01
影响因子：
3
通讯作者：
Lopez Jornet, Pia
中科院分区：
文献类型：
--
作者：
Pons-Fuster Lopez, Eduardo;Seoane Leston, Juan;Lopez Jornet, Pia
目的：分析唑来膦酸（zoledronic acid，ZA）单独及与表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate，EGCG）联合应用对人成骨细胞的作用（0.01-10 μ M）的ZA或ZA + EGCG进行细胞活力、矿化、胶原合成和细胞迁移试验。微摩尔（1和5 μ M）剂量的ZA降低细胞活力（p < 0.001）、矿化（p < 0.001）和胶原合成（p < 0.001），而纳摩尔剂量（0.1 μ M）不产生任何影响。应用表没食子酸没食子酸酯部分逆转了ZA产生的抑制作用，甚至增加了矿化作用（p < 0.001）。结论：获得的数据表明，微摩尔剂量的ZA对成骨细胞的所有测量参数均产生负面影响，而纳摩尔水平的ZA仅影响细胞迁移，对成骨细胞没有其他影响。添加EGCG部分逆转了ZA产生的作用，表明这可能是一种有希望的预防BRONJ的措施。
Objective: analyze the effect of zoledronic acid (ZA) alone and in combination with epigallocatechin-3-gallate (EGCG) on human osteoblast cells.Methods: SAOS-2 osteoblastic phenotype cell line treated with different doses (0.01-10 mu M) of ZA or ZA + EGCG underwent cell viability, mineralization, collagen synthesis and cell migration tests.Results: Micromolar (1 and 5 mu M) doses of ZA reduced cell viability (p < 0.001), mineralization (p < 0.001), and collagen synthesis (p < 0.001), while nanomolar doses (0.1 mu M) did not produce any effect. EGCG application partly reversed the inhibition produced by ZA, and even increased mineralization (p < 0.001).Conclusions: the data obtained shows that ZA at micromolar doses produces negative effects on osteoblast at all parameters measured, while at nanomolar levels ZA only affected cell migration and had no other effects on osteoblasts. The addition of EGCG partly reversed the effects produced by ZA, suggesting that this might be a promising preventative measure against BRONJ.