LIMITED HETEROGENEITY OF T-CELL RECEPTOR V-BETA GENE-EXPRESSION IN THE EARLY-STAGE OF INSULITIS IN NOD MICE
LIMITED HETEROGENEITY OF T-CELL RECEPTOR V-BETA GENE-EXPRESSION IN THE EARLY-STAGE OF INSULITIS IN NOD MICE
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DOI：
10.1016/0165-2478(93)90030-6
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发表时间：
1993-08-01
影响因子：
4.4
通讯作者：
HURTENBACH, U
中科院分区：
文献类型：
--
作者：
DREXLER, K;BURTLES, S;HURTENBACH, U
非肥胖型糖尿病（NOD）小鼠自发发展为I型糖尿病。这种疾病始于T细胞浸润朗格汉斯岛。因此，我们采用Vbeta1-Vbeta17特异性寡核苷酸聚合酶链反应（PCR）检测了4 - 10周龄雌性NOD小鼠胰岛浸润的t细胞受体（TCR） Vbeta区。该研究显示，TCR Vbeta转录本的异质性有限，在4周龄的胰岛素发病时，Vbeta1转录本占主导地位。Vbeta1 PCR片段的TCR VDJbeta序列在大多数小鼠个体中是相同的。在几种不同的小鼠中，检测到β连接区域的相似性。相比之下，TCR Vbeta的使用在晚期胰岛素小鼠（即从6周龄开始）中存在很大的异质性。因此，这些数据表明TCR Vbeta的使用具有有限的异质性，在疾病的起始阶段以Vtbeta1为主。
Non-obese-diabetic (NOD) mice spontaneously develop type I diabetes. The disease starts with T cells infiltrating the islets of Langerhans. We therefore examined the T-cell receptor (TCR) Vbeta region repertoire in islet infiltrates from individual female NOD mice from 4 to 10 week old by polymerase chain reaction (PCR) using Vbeta1-Vbeta17 specific oligonucleotides. The study revealed a limited heterogeneity of TCR Vbeta transcripts with a predominance of Vbeta1 at the onset of insulitis, i.e. at 4 weeks of age. The TCR VDJbeta sequences of the Vbeta1 PCR fragments were identical in most of the individual mice. Among several different mice, similarities in the beta-junctional regions were detected. In contrast, a large heterogeneity of TCR Vbeta usage was found in mice with advanced insulitis, i.e., from 6 weeks of age on. Thus, these data suggest a limited heterogencity of TCR Vbeta usage with a predominance of Vtbeta1 at the initiation phase of the disease.