Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing
Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing
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DOI：
10.1016/j.biomaterials.2009.06.033
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发表时间：
2009-10-01
期刊：
影响因子：
14
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Hannachi, Imen Elloumi;Itoga, Kazuyoshi;Okano, Teruo
本研究的目的是开发一种用于组织工程的可转移微图案细胞片的新方法。为了实现这一发展，纤维连接蛋白的微接触印刷在市售的温度响应盘被采用。将原代大鼠肝细胞接种于纤维连接蛋白打印的培养皿表面。在无血清条件下，肝细胞选择性地附着在纤维连接蛋白结构域上。然后，第二种细胞类型的内皮细胞在血清的存在下播种。双荧光染色显示内皮细胞成功粘附在肝细胞结构域的间隙上。最后，所有细胞在温度降低后被收获为一个连续的微图案细胞片。利用细胞片操纵器，用明胶层支撑收获的细胞片，收获的微图案细胞片被转移到新的培养皿表面。该技术可用于制造具有复杂微结构的厚组织结构。2009爱思唯尔有限公司版权所有。
The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture. (C) 2009 Elsevier Ltd. All rights reserved.