Protease nexin-2/amyloid beta-protein precursor in blood is a platelet-specific protein.
Protease nexin-2/amyloid beta-protein precursor in blood is a platelet-specific protein.
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血液中的蛋白酶 nexin-2/淀粉样β蛋白前体是一种血小板特异性蛋白。
DOI：
10.1016/s0006-291x(05)81193-3
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发表时间：
1991
影响因子：
3.1
通讯作者：
Cunningham,DD
中科院分区：
文献类型：
--
作者：
VanNostrand,WE;Schmaier,AH;Farrow,JS;Cines,DB;Cunningham,DD
蛋白酶抑制剂蛋白酶连接蛋白-2（PN-2）是淀粉样β蛋白前体（APP）的分泌形式，其含有Kunitz蛋白酶抑制剂结构域。PN 2/APP是血小板活化后分泌的一种丰富的血小板α颗粒蛋白。PN 2/APP mRNA存在于培养的内皮细胞中，并且在血浆中检测到该蛋白。在本研究中，我们定量了血小板、血浆和血管系统的几种不同细胞类型中的PN-2/APP，以确定蛋白质在循环系统中的储存库。我们报告，PN-2/APP主要是在血管室的血小板蛋白。未受刺激的脐静脉内皮细胞，粒细胞或单核细胞的裂解液含有很少的PN-2/APP基于敏感的功能蛋白酶结合和免疫印迹分析。定量免疫印迹研究表明，正常枸橼酸血浆中含有≤ 60 pM PN-2/APP。相反，血小板可贡献高达30 nM PN-2/APP，表明它们是血液中蛋白质的主要来源。
The protease inhibitor, protease nexin-2 (PN-2), is the secreted form of the amyloid β-protein precursor (APP) which contains the Kunitz protease inhibitor domain. PN2/APP is an abundant platelet α-granule protein which is secreted upon platelet activation. PN2/APP mRNA is present in cultured endothelial cells and the protein has been detected in plasma. In the present studies we quantitated PN-2/APP in platelets, plasma and several different cell types of the vasculature to identify the repository of the protein in the circulatory system. We report that PN-2/APP is predominantly a platelet protein in the vascular compartment. Lysates of unstimulated umbilical vein endothelial cells, granulocytes or monocytes contained little PN-2/APP based on sensitive functional protease binding and immunoblotting assays. Quantitative immunoblotting studies demonstrated that normal citrated-plasma contains ≤ 60 pM PN-2/APP. In contrast, platelets can contribute up to 30 nM PN-2/APP, indicating that they are the major source of the protein in blood.