Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis
Laser-induced fluorescence detector with a fiber-coupled micro GRIN lens for capillary electrophoresis
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DOI：
10.1364/ao.391661
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发表时间：
2020-06-01
期刊：
影响因子：
1.9
通讯作者：
Dada, Oluwatosin O.
中科院分区：
文献类型：
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作者：
Dada, Oluwatosin O.
毛细管电泳结合鞘流激光诱导荧光（LIF）检测已被证明对化学和生化分析具有出色的灵敏度。然而，一个主要的缺点仍然是传统上采用的光学结构的复杂性。本文提出了一种基于光纤和微梯度折射率（GRIN）透镜的简化共聚焦光学，用于激光诱导荧光毛细管电泳的模块化光学设计。我们演示了使用光学系统与鞘流试管作为毛细管电泳激光诱导荧光检测器。荧光素钠标准样品的浓度检出限为8 +/- 2 pM，质量检出限为57 zeptomole。(C) 2020美国光学学会
Capillary electrophoresis coupled with sheath-flow laser-induced fluorescence (LIF) detection has been shown to offer outstanding sensitivity for chemical and biochemical analysis. However, a major drawback remains with the complexity of the optical configuration traditionally employed. Here we present a simplified confocal optics based on fiber optics and micro gradient-index (GRIN) lenses for modular optical design in capillary electrophoresis with laser-induced fluorescence. We demonstrate the use of the optical system with a sheath-flow cuvette as the laser-induced fluorescence detector for capillary electrophoresis. The system's performance was established with concentration detection limits of 8 +/- 2 pM and mass detection limits of 57 zeptomole for a standard sodium fluorescein sample. (C) 2020 Optical Society of America