Ethnobotanical survey and cytotoxicity testing of plants of South-western Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp leaves
Ethnobotanical survey and cytotoxicity testing of plants of South-western Nigeria used to treat cancer, with isolation of cytotoxic constituents from Cajanus cajan Millsp leaves
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DOI：
10.1016/j.jep.2010.01.009
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发表时间：
2010-03-24
影响因子：
5.4
通讯作者：
Efferth, T.
中科院分区：
文献类型：
--
作者：
Ashidi, J. S.;Houghton, P. J.;Efferth, T.
民族药理学的相关性只有很少的文献对抗癌成分的药用植物从尼日利亚，但传统的治疗师在该地区的研究声称，已管理疾病的病人与一些成功的使用物种研究的目的。为了记录在西南部尼日利亚常用于治疗癌症的植物，并使用体外细胞毒性试验来测试索赔的科学依据。方法使用结构化问卷来探索传统治疗者之间的民族植物学实践。使用磺酰罗丹明B（SR B）试验在暴露和恢复实验中筛选最常见物种的甲醇提取物的细胞毒性。人乳腺癌细胞MCF-7。人大细胞肺癌细胞系COR-L23和人无黑色素黑素瘤C32）和一种正常细胞系（正常人角质形成细胞SVK-14）用于筛选提取物和获得的级分。木豆的提取物显示出相当大的活性，并进一步分配，二氯甲烷级分进行制备层析，得到六种化合物十六烷酸甲酯、香树素、β-谷甾醇、球松素、长柱木素A和长柱木素C。此外，阿霉素敏感的急性T淋巴细胞白血病细胞系（CCRF-CEM）及其多药耐药亚系（CEM/ADR 5000）用于XTT试验，以评价所获得的纯化合物的活性。记录了45种药用植物及其药用部位的地方名称在5个物种中观察到（IC 50值小于50 μ g/mL）（硬叶木豆、孜然木豆、巴戟天睡莲和安哥拉密花）硬叶木豆和木豆的活性最强。木豆的二氯甲烷级分的IC 50值为5-10 μ g/mL，其中2种芪类成分longistylins A和C是主要的作用成分，其对癌细胞系的IC_（50）值为0.7 - 14.7 μ M。结论所测试的大多数物种对癌细胞系具有一定的细胞毒作用，这在一定程度上支持了它们在治疗癌症的草药制剂中的传统包含，但观察到对癌细胞的选择性很小，这引起了对它们在传统治疗中的安全性和有效性的关注。长柱木豆素A和C似乎是木豆提取物的大部分活性的原因。(C)2010爱思唯尔爱尔兰有限公司版权所有
Ethnopharmacological relevance There is only scant literature on the anticancer components of medicinal plants from Nigeria, yet traditional healers in the area under study claim to have been managing the disease in their patients with some success using the species studiedAim of study. To document plants commonly used to treat cancer in South-western Nigeria and to test the scientific basis of the claims using in vitro cytotoxicity tests.Methods Structured questionnaires were used to explore the ethnobotanical practices amongst the traditional healers Methanol extracts of the most common species cited were screened for cytotoxicity using the sulforhodamine B (SRB) assay in both exposure and recovery experiments Three cancer cell lines (human breast adenocarcinoma cell line MCF-7. human large cell lung carcinoma cell line COR-L23 and human amelanotic melanoma C32) and one normal cell line (normal human keratinocytes SVK-14) were used for the screening of the extracts and the fractions obtained. The extract of Cajanus cajan showed considerable activity and was further partitioned and the dichloromethane fraction was subjected to preparative chomatography to yield six compounds hexadecanoic acid methyl ester, aamyrin, beta-sitosterol, pinostrobin, longistylin A and longistylin C. Pinostrobm and longistylins A and C were tested for cytotoxicity on the cancer cell lines In addition, an adriamycin-sensitive acute T-lymphoblastic leukaemia cell line (CCRF-CEM) and its multidrug-resistant sub-line (CEM/ADR5000) were used in an XTT assay to evaluate the activity of the pure compounds obtainedResults A total of 30 healers from S W Nigeria were involved in the study. 45 species were recorded with their local names with parts used in the traditional therapeutic preparations Cytotoxicity (IC50 values less than 50 mu g/mL) was observed in 5 species (Acanthospermum hispidum, Cumin's cajan, Morinda Nymphaea lotus and Pycnanthus angolensis) Acanthospermum hispidum and Cajanus cajan were the most active The dichloromethane fraction of Cajanus cajan had IC50 value 5-10 mu g/mL, with the two constituent stilbenes, longistylins A and C, being primarily responsible, with IC50 values of 0 7-14 7 mu M against the range of cancer cell linesConclusions Most of the species tested had some cytotoxic effect on the cancer cell lines, which to some extent supports their traditional inclusion in herbal preparations for treatment of cancer However, little selectivity for cancer cells was observed, which raises concerns over their safety and efficacy in traditional treatment The longistylins A and C appear to be responsible for much of the activity of Cajanus man extract. (C) 2010 Elsevier Ireland Ltd. All rights reserved