Neurogenic vasodilation and release of calcitonin gene-related peptide (CGRP) from perivascular nerves in the rat mesenteric artery.
Neurogenic vasodilation and release of calcitonin gene-related peptide (CGRP) from perivascular nerves in the rat mesenteric artery.
复制标题
大鼠肠系膜动脉血管周围神经的神经源性血管舒张和降钙素基因相关肽（CGRP）的释放。
DOI：
10.1016/0006-291x(89)92758-7
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
K. Goto
中科院分区：
文献类型：
--
作者：
Akira Fujimori;Akira Saito;Sadao Kimura;Tsuyoshi Watanabe;Yasuo Uchiyama;H. Kawasaki;K. Goto
用放射免疫法（RIA）观察了血管周围神经刺激（PNS）对离体大鼠肠系膜动脉降钙素基因相关肽（CGRP）释放的影响。放免和高效液相色谱联用分析表明，释放的CGRP样免疫反应物（CGRP-LI）是CGRP本身及其氧化形式。CGRP阳性神经定位于肠系膜大动脉及其分支的血管周围神经。在甲氧胺预收缩的标本中，用含有肾上腺素能神经元阻断剂乙啶的溶液灌流，PNS引起血管舒张反应和灌流液中CGRP-LI的增加，呈频率依赖性。这两种反应都被河豚毒素（10− 6 M）减弱，表明它们起源于神经源性。灌流液中Ca ~（2+）的去除也可抑制PNS诱导的CGRP-LI的释放。提示CGRP在大鼠肠系膜血管床神经源性舒张中起递质作用。
The effect of perivascular nerve stimulation (PNS) on the release of calcitonin gene-related peptide (CGRP) was examined by radioimmunoassay (RIA) in isolated, perfused rat mesenteric arteries. The released CGRP-like immunoreactivity (CGRP-LI) was identified to be CGRP itself and its oxidized form by combined analysis with RIA and high performance liquid chromatography. CGRP-LI was localized in the perivascular nerves of the large mesenteric artery and its branches. In the preparation precontracted by methoxamine, and perfused with a solution containing guanethidine, an adrenergic neuron blocker, PNS induced vasodilator responses and an increase of CGRP-LI in the perfusate in a frequency-dependent manner. Both the responses were attenuated by tetrodotoxin (10−6M), suggesting that they were neurogenic in origin. Removal of Ca2+from the perfusing solution also abolished the PNS-induced release of CGRP-LI. These findings suggest that CGRP plays a transmitter role in the neurogenic vasodilation in the rat mesenteric vascular bed.
大型猫脑动脉的胆碱能机制。
影响因子：
20.1
作者：
Lee,TJ
通讯作者：
Lee,TJ
猫大脑动脉中降钙素基因相关肽和血管舒张神经。
DOI：
--
发表时间：
1989
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
Saito,A;Masaki,T;Uchiyama,Y;Lee,TJ;Goto,K
通讯作者：
Goto,K