Long-term storage of DNA-free RNA for use in vaccine studies
Long-term storage of DNA-free RNA for use in vaccine studies
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DOI：
10.2144/000112593
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发表时间：
2007-11-01
期刊：
影响因子：
2.7
通讯作者：
Gowans, Eric J.
中科院分区：
文献类型：
--
作者：
Jones, Kathryn L.;Drane, Debbie;Gowans, Eric J.
RNA复制子是潜在的疫苗递送载体，但被认为用于此类用途时稳定性太差。本研究检测了编码丙型肝炎病毒（HCV）蛋白质的体外转录复制子RNA的回收率、完整性和功能。为了去除残留的模板DNA，先用TURBO DNase消化RNA，接着用RNeasy DNase试剂盒处理，并通过RNeasy柱纯化。将RNA在蒸馏水或海藻糖中冻干，在氮气下储存长达10个月，并在不同时间点进行分析。在……储存的RNA的回收率
RNA replicons represent potential vaccine delivery vehicles, but are considered too unstable for such use. This study examined the recovery, integrity and function of in vitro transcribed replicon RNA encoding hepatitis C virus (HCV) proteins. To remove residual template DNA, the RNA was digested with TURBO DNase followed by RNeasy DNase set and purified through an RNeasy column. The RNA was freeze-dried in distilled water or trehalose, stored under nitrogen gas for up to 10 months and analyzed at different time points. The recovery of RNA stored at