Effects of calcium on transendothelial albumin transfer and electrical resistance.
Effects of calcium on transendothelial albumin transfer and electrical resistance.
复制标题
钙对跨内皮白蛋白转运和电阻的影响。
DOI：
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发表时间：
1986
影响因子：
3.3
通讯作者：
S. Shasby
中科院分区：
文献类型：
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作者：
D. Shasby;S. Shasby
Nicolaysen，以及最近的Kern和Malik报告说，钙的螯合作用增加了隔离灌流肺中微血管的水力传导性和白蛋白通透性。为了开始了解钙如何影响内皮功能，我们研究了钙离子螯合对体外培养的内皮细胞的影响。用乙二醇双(β-氨基乙醚)-N，N‘-四乙酸络合钙，可使血管内皮细胞白蛋白转移率提高125%。在含钙的培养液中重新孵育内皮细胞，使其转移率恢复到原来的值。细胞外钙离子的螯合作用可消除跨内皮细胞的电阻。内皮细胞在含钙的培养液中重新孵育后，跨内皮细胞的电阻也恢复到正常水平。细胞外钙离子的螯合作用导致相邻内皮细胞相互收缩，在补钙的培养液中重新孵育后，相邻细胞的正常结合得以恢复。细胞外钙离子的络合作用使单个内皮细胞肌动蛋白的外周条带向心性回缩，在含钙的培养液中重新孵育后，肌动蛋白条带恢复到正常位置。钙是内皮完整性的重要决定因素，钙的变化会导致内皮屏障特性和内皮细胞形状的动态变化。
Nicolaysen, and more recently Kern and Malik, reported that chelation of calcium increased microvascular hydraulic conductivity and albumin permeability in isolated perfused lungs. To begin to understand how calcium affects endothelial function we examined the effect of calcium chelation on an in vitro endothelium. Chelation of calcium with ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid increased the rate of transendothelial albumin transfer by 125%. Reincubation of the endothelium in calcium-repleted medium restored the rate of transfer to its original value. Chelation of extracellular calcium abolished transendothelial electrical resistance. The transendothelial electrical resistance was also restored to normal by reincubation of the endothelium in calcium-repleted medium. Chelation of extracellular calcium caused adjacent endothelial cells to retract from one another, and normal apposition of adjacent cells was restored after reincubation in calcium-repleted medium. Chelation of extracellular calcium produced a centripetal retraction of the peripheral band of actin in individual endothelial cells, and the actin band resumed its normal location after reincubation in calcium-repleted medium. Calcium is an important determinant of endothelial integrity and alterations in calcium produce dynamic changes in endothelial barrier properties and in endothelial-cell shape.