The phosphotriesterase gene opdA in Agrobacterium radiobacter P230 is transposable
The phosphotriesterase gene opdA in Agrobacterium radiobacter P230 is transposable
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DOI：
10.1016/s0378-1097(03)00211-8
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发表时间：
2003-05-16
影响因子：
2.1
通讯作者：
Oakeshott, JG
中科院分区：
文献类型：
--
作者：
Horne, I;Qiu, XH;Oakeshott, JG
我们报道了农杆菌P230的opdA磷酸三酯酶基因上游的一个转座酶基因(TnpA)，以及在这两个基因两侧的指示插入序列的反向重复序列。TnpA基因和反向重复序列都与偶发分枝杆菌的Tn610转座子相似。另外两个假定的开放阅读框将opdA和tnpA分开，推测的翻译产物与嗜热脂肪地质杆菌IS5376转座子上编码的两个蛋白质相似。为了验证转座子上含有这些基因这一命题，我们构建了一个人工复合转座子。然后将这个人工转座子导入到大肠杆菌DH10beta细胞中。在大肠杆菌染色体上存在opdA，反向聚合酶链式反应证实了插入。目前的数据表明，转座在opdlopdA基因在广泛的土壤细菌中的分布中可能起到了作用。(C)2003年欧洲微生物学会联合会。爱思唯尔科学公司出版。版权所有。
We report a transposase gene (tnpA) upstream of the opdA phosphotriesterase gene of Agrobacterium radiobacter P230, as well as inverted repeats indicative of insertion sequences, flanking the two genes. Both the tnpA gene and the inverted repeats resemble the Tn610 transposon from Mycobacterium fortuitum. Two additional putative open reading frames separate opdA and tnpA with inferred translation products with similarity to two proteins encoded on the Geobacillus stearothermophilus IS5376 transposon. To test the proposition that these genes were contained on a transposon, an artificial composite transposon was constructed. This artificial transposon was then delivered into Escherichia coli DH10beta cells. Transposition was demonstrated by the presence of opdA on the E. coli chromosome and confirmation of insertion by inverse polymerase chain reaction. The data presented suggest a possible role of transposition in the distribution of the opdlopdA genes across a wide range of soil bacteria. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.