A multi-tasking polypeptide from bloodworm jaws: Catalyst, template, and copolymer in film formation
A multi-tasking polypeptide from bloodworm jaws: Catalyst, template, and copolymer in film formation
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DOI：
10.1016/j.matt.2022.04.001
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发表时间：
2022-06-01
期刊：
影响因子：
18.9
通讯作者：
Waite, J. Herbert
中科院分区：
文献类型：
--
作者：
Wonderly, William R.;Nguyen, Tuan T. D.;Waite, J. Herbert
血吸虫的颌骨是蛋白质、黑色素以及矿物质和离子铜的复合体。到目前为止，纳米机械测试已经将离子铜和黑色素与硬度和耐磨性联系起来，但蛋白质的功能尚不确定。在这里，我们描述了一种名为多任务蛋白(MTP)的富含甘氨酸和组氨酸的蛋白质，并使用重组蛋白证明了它对颌骨的形成和性能具有六种不同的功能，即(1)招募22当量的Cu2+，(2)调节MTP-铜络合物的液-液相分离，(3)催化3，4-二羟基苯丙氨酸(DOPA)的氧化为黑色素，(4)模板黑色素的界面聚合，(5)将黑色素及其自身整合到薄膜和纤维中，以及(6)通过铜桥提供分子间的凝聚力。MTP通过承担前所未有的构建块、组织者和制造者的角色来实现所有这些-这一加工壮举与其他聚合物复合材料、共混物和/或网络的自主生产具有相当大的相关性。
Bloodworm jaws are composites of protein, melanin, and both mineral and ionic copper. To date, nanomechanical tests have correlated the ionic copper and melanin with hardness and wear resistance, but the function of protein is uncertain. Here, we characterize a Gly-and His-rich protein called multi-tasking protein (MTP) and, using recombinant protein, show that it performs six distinct functions critical for jaw formation and performance, namely (1) recruiting 22 equiv of Cu2+, (2) mediating a liquid-liquid phase separation of the MTP-copper complex, (3) catalyzing the oxidation of 3,4-dihydroxyphenylalanine (Dopa) to melanin, (4) templating the interfacial polymerization of melanin, (5) integrating melanin and itself into thin films and fibers, and (6) providing intermolecular cohesion through Cu bridging. MTP achieves all these by assuming unprecedented roles as a building block, organizer, and fabricator-a processing feat of considerable relevance to the autonomous production of other polymer composites, blends, and/or networks.