Regeneration of dental pulp after pulpotomy by transplantation of CD31-/CD146- side population cells from a canine tooth
Regeneration of dental pulp after pulpotomy by transplantation of CD31-/CD146- side population cells from a canine tooth
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DOI：
10.2217/rme.09.5
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发表时间：
2009-05-01
影响因子：
2.7
通讯作者：
Nakashima, Misako
中科院分区：
文献类型：
--
作者：
Iohara, Koichiro;Zheng, Li;Nakashima, Misako
目标：目的：从犬牙髓侧群（SP）细胞中提取干/祖细胞，在体内实现牙髓的完全再生。材料与方法：应用流式细胞仪从犬牙髓中分离出SP细胞亚群CD 31（-）/CD 146（-）SP细胞。在狗牙髓损伤的实验模型中评价了SP细胞的这种亚组分的效率。结果：分离的SP细胞在体外形成广泛的管状网络结构。将SP细胞移植到离断牙髓的体内模型中，在14天内导致具有毛细血管和神经元细胞的牙髓组织完全再生。基因表达研究表明促血管生成因子的表达，这意味着对内皮细胞的营养作用。结论：这项调查表明，潜在的效用分级SP细胞作为一个来源的细胞的总牙髓再生完成血管生成和血管生成。
Aim: To achieve complete regeneration of dental pulp in vivo by stem/progenitor cells obtained from a fraction of side population (SP) cells from canine pulp. Materials & methods: A subfraction of SP cells, CD31(-)/CD146(-)SP cells, were isolated by flow cytometry from canine dental pulp. The efficiency of this subfraction of SP cells was evaluated in an experimental model of pulp injury in the dog. Results: The fractionated SP cells formed extensive networks of tube-like structures in vitro. Transplantation of the SP cells into an in vivo model of amputated pulp resulted in complete regeneration of pulp tissue with capillaries and neuronal cells within 14 days. Gene-expression studies demonstrated the expression of pro-angiogenic factors, implying trophic action on endothelial cells. Conclusions: This investigation demonstrates the potential utility of fractionated SP cells as a source of cells for total pulp regeneration complete with angiogenesis and vasculogenesis.