Identification of changes in the volatile compounds of robusta coffee beans during drying based on HS-SPME/GC-MS and E-nose analyses with the aid of chemometrics
Identification of changes in the volatile compounds of robusta coffee beans during drying based on HS-SPME/GC-MS and E-nose analyses with the aid of chemometrics
复制标题
基于HS-SPME/GC-MS和化学计量学电子鼻分析鉴定罗布斯塔咖啡豆干燥过程中挥发性化合物的变化
DOI：
10.1016/j.lwt.2022.113317
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发表时间：
2022-03-21
影响因子：
6
通讯作者：
Zhang, Zhenzhen
中科院分区：
文献类型：
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作者：
Zhang, Ke;Cheng, Jinhuan;Zhang, Zhenzhen
采用顶空固相微萃取/气相色谱-质谱（HS-SPME/GC-MS）和电子鼻（E-nose）分析技术，研究罗巴斯塔咖啡在热风干燥过程中的综合风味特征。HS-SPME/GC-MS共鉴定出62种挥发性化合物，其中酸、醛、醇、酯的含量在干燥过程中有所下降，而烃类和酸类的相对含量则有所上升。主成分分析的电子鼻结果清楚地区分了与干燥时间相关的样品，发现初始阶段（0 h，3 h和6 h）的样品与不同的传感器具有强阳性。偏最小二乘判别分析（PLS-DA）表明，醇类，酮类和酯类是咖啡豆风味的主要贡献化合物。PLS-DA的VIP评分大于1说明T30/1、T70/2、PA/2和P30/2与酮类、含硫化合物和酯类呈阳性。研究结果可为咖啡豆干燥过程中挥发性成分的变化规律提供理论依据，并可应用于其他热敏性热带农产品。
In this study, headspace solid-phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) and electronic-nose (E-nose) analyses were performed to examine the comprehensive flavor profiles of robusta coffee during heat air drying. A total of 62 volatile compounds were identified by HS-SPME/GC-MS, the level of acids, aldehydes, alcohols and esters decreased during drying process, however, the relative content of hydrocarbons and acids increased. Principal component analysis of the E-nose results clearly differentiated the samples related to drying time, and it was found that samples of initial stages (0 h, 3 h, and 6 h) had strong positive with different sensors. Partial least squares-discriminant analysis (PLS-DA) indicated that alcohols, ketones, and esters were the main contributing compounds to the flavour of coffee beans. The VIP score of PLS-DA larger than one illustrated that T30/1, T70/2, PA/2, and P30/2 were positive with ketones, sulfur compounds, and esters. These results could provide theoretical evidence about the change rule in volatile compounds of coffee beans during drying process, and could be applied to other thermally sensitive tropical agricultural products.