A Small-Volume, Low-Cost, and Versatile Continuous Culture Device.
A Small-Volume, Low-Cost, and Versatile Continuous Culture Device.
复制标题
小体积，低成本和多功能的连续培养装置。
DOI：
10.1371/journal.pone.0133384
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Rodrigue S
中科院分区：
文献类型：
--
作者：
Matteau D;Baby V;Pelletier S;Rodrigue S
连续培养装置可用于多种目的，例如建立可重复的生长条件或在恒定环境下长期维持细胞群。然而，市售仪器价格昂贵，并非设计用于处理毫升范围内的小体积，并且可能缺乏满足多个实验室的不同实验需求所需的灵活性。我们开发了一种多功能的连续培养系统，并为潜在用户提供详细的说明以及图形用户界面软件来组装和操作自己的仪器。所提出的配置可以同时控制三个培养室，并且所有组件都可以从各种来源轻松获得。我们证明我们的连续培养装置可以在不同模式下使用，并且可以轻松编程以充当浊度调节器或恒化器。向培养容器中添加新鲜培养基可以通过实时反馈回路控制，或者简单地校准以提供规定的体积。此外，所选的发光二极管和光电探测器可以使用酚红作为 pH 指示剂，可用于间接监测细胞群的大量代谢活动而不是浊度。这种经济实惠且可定制的系统将成为生物学许多领域（例如微生物生态学以及系统和合成生物学）的有用工具。
Continuous culture devices can be used for various purposes such as establishing reproducible growth conditions or maintaining cell populations under a constant environment for long periods. However, commercially available instruments are expensive, were not designed to handle small volumes in the milliliter range, and can lack the flexibility required for the diverse experimental needs found in several laboratories. We developed a versatile continuous culture system and provide detailed instructions as well as a graphical user interface software for potential users to assemble and operate their own instrument. Three culture chambers can be controlled simultaneously with the proposed configuration, and all components are readily available from various sources. We demonstrate that our continuous culture device can be used under different modes, and can easily be programmed to behave either as a turbidostat or chemostat. Addition of fresh medium to the culture vessel can be controlled by a real-time feedback loop or simply calibrated to deliver a defined volume. Furthermore, the selected light-emitting diode and photodetector enable the use of phenol red as a pH indicator, which can be used to indirectly monitor the bulk metabolic activity of a cell population rather than the turbidity. This affordable and customizable system will constitute a useful tool in many areas of biology such as microbial ecology as well as systems and synthetic biology.