Genetic characterization of peste des petits ruminants virus, Turkey, 2009-2013
Genetic characterization of peste des petits ruminants virus, Turkey, 2009-2013
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DOI：
10.1016/j.rvsc.2015.05.005
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发表时间：
2015-08-01
影响因子：
2.4
通讯作者：
Sait, Ahmet
中科院分区：
文献类型：
--
作者：
Sevik, Murat;Sait, Ahmet
小反刍兽疫是土耳其小反刍动物的一种地方病，疫苗接种一直是控制方法，但也有零星暴发的报告。本研究通过测序融合(F)蛋白和核蛋白(N)基因片段并进行系统发育分析，对当地小反刍兽疫毒（PPRV）进行鉴定，重点研究田间病毒的遗传变异。在2009-2013年期间，从土耳其中部和地中海地区临床怀疑感染PPRV的绵羊和山羊中采集了样本。基于F基因序列的系统发育分析表明，本研究的野外分离株与其他中东分离株属于谱系4。虽然N基因序列显示了不同的模式，但本研究中的野外分离株与先前的土耳其分离株聚集在一起，这可能代表了PPRV分子流行病学的真实情况。(C) 2015 Elsevier Ltd.版权所有。
Peste des petits ruminants is an endemic disease of small ruminants in Turkey and vaccination has been the method of control but sporadic outbreaks have been reported. This study was carried out to characterize the local peste des petits ruminants virus (PPRV) by sequencing fusion (F) protein and nucleoprotein (N) gene segments and phylogenetic analysis, so as to focus on genetic variation in the field viruses. Samples were collected from sheep and goats clinically suspected of having PPRV infection in Central and Mediterranean regions of Turkey during 2009-2013. Phylogenetic analysis based on the F gene sequences showed that the field isolates in the present study belong to lineage 4 with other Middle East isolates. While N gene sequences revealed a different pattern, the field isolates in the present study clustered with previous Turkish isolates, which probably represents the true picture of molecular epidemiology for PPRV. (C) 2015 Elsevier Ltd. All rights reserved.