Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column
Rapid simultaneous determination of isoflavones in Radix puerariae using high-performance liquid chromatography-triple quadrupole mass spectrometry with novel shell-type column
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DOI：
10.1002/jssc.201100295
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发表时间：
2011-10-01
影响因子：
3.1
通讯作者：
Wang, Yitao
中科院分区：
文献类型：
--
作者：
Du, Gang;Zhao, Haiyu;Wang, Yitao
建立了高效液相色谱-三重四极杆质谱联用（HPLC-MS/MS）快速测定葛根中13种葛根素的方法。采用Kinetex核壳C-18柱（50 mm × 2.1 mm id，2.6 μ m），采用梯度洗脱。通过与相关对照品的保留时间和质谱数据比较，确定了13个化合物的色谱峰。采用多反应监测（MRM）负离子模式进行定量分析。所有标准曲线在检测范围内线性关系良好（r（2）>0.9990）。柱上检出限和定量限分别低于0.017和0.873 μ g/mL。13种分析物的日内和日间精密度为
A high-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (MS/MS) method was developed for rapid determination of 13 isoflavones in Radix puerariae. A novel shell-type column, namely Kinetex core-shell C-18 column (50 mm x 2.1 mm id, 2.6 mu m), and gradient elution were used during the analysis. The chromatographic peaks of 13 investigated compounds were identified by comparing their retention time and MS data with the related reference compounds. Multiple-reaction monitoring (MRM) was employed for the quantitative analysis with negative ionization mode. All calibration curves showed good linearity (r(2)>0.9990) within test ranges. The LOD and LOQ were lower than 0.017 and 0.873 mu g/mL on column, respectively. The intra- and inter-day precisions for 13 analytes were