Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus.
Isolation and structure of pro-somatostatin: a putative somatostatin precursor from pig hypothalamus.
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生长抑素原的分离和结构：来自猪下丘脑的假定生长抑素前体。
DOI：
10.1073/pnas.77.8.4489
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发表时间：
1980
影响因子：
11.1
通讯作者：
Hood,LE
中科院分区：
文献类型：
--
作者：
Schally,AV;Huang,WY;Chang,RC;Arimura,A;Redding,TW;Millar,RP;Hunkapiller,MW;Hood,LE
我们从猪下丘脑的酸提取物中分离出一种八十二肽，我们将其命名为生长抑素原，并发现其具有氨基酸序列Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys。这种二十八肽在体外具有高生长激素（生长激素）和催乳素释放抑制活性。它还与针对生长抑素十四肽产生的抗血清发生强烈交叉反应。这种八十二肽很可能是下丘脑中生长抑素的前体（激素原）。还观察到生长抑素的分子尺寸更大的前体的存在。
An octacosapeptide that we named pro-somatostatin has been isolated from acid extracts of porcine hypothalami and found to have the amino acid sequence Ser-Ala-Asn-Ser-Asn-Pro-Ala-Met-Ala-Pro-Arg-Glu-Arg-Lys-Ala-Gly-Cys-Lys-Asn-Phe-Phe-Trp-Lys-Thr-Phe-Thr-Ser-Cys. This octacosapeptide possesses high somatotropin (growth hormone) and prolactin release-inhibiting activity in vitro. It also crossreacts strongly with antisera generated against the somatostatin tetradecapeptide. This octacosapeptide is most likely a precursor (pro-hormone) of somatostatin in the hypothalamus. The existence of still larger molecular size precursors of somatostatin was also observed.