IN THIAMINE-DEFICIENCY, ACTIVATION OF ERYTHROCYTE TRANSKETOLASE BY THIAMINE INVIVO EXCEEDS ACTIVATION BY COFACTOR INVITRO
IN THIAMINE-DEFICIENCY, ACTIVATION OF ERYTHROCYTE TRANSKETOLASE BY THIAMINE INVIVO EXCEEDS ACTIVATION BY COFACTOR INVITRO
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DOI：
10.1016/0009-8981(91)90253-9
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发表时间：
1991-10-14
影响因子：
5
通讯作者：
NIXON, PF
中科院分区：
文献类型：
--
作者：
PRICE, J;CLAGUE, AE;NIXON, PF
在 60 名硫胺素缺乏患者中，硫胺素治疗前，体外被硫胺素二磷酸辅因子激活后的平均红细胞转酮醇酶活性（代表全酶和脱辅基酶活性的表观总和）为 0.609 (SD 0.166) U/g Hb，在给患者施用硫胺素后立即升至 0.772 (SD 0.152) U/g Hb。这些值之间的差值 0.163 (SD 0.130) U/g 是转酮醇酶蛋白的平均活性，转酮酶蛋白在体内可以被硫胺素激活，但在体外不能被二磷酸硫胺素激活。这种差异与这些患者的初始红细胞转酮酶活性较低有关，但与他们的酒精摄入量、肝功能或诊断无关。
In 60 thiamine deficient patients, the mean erythrocyte transketolase activity after activation by thiamine diphosphate cofactor in vitro, representing the apparent sum of holoenzyme and apoenzyme activities, was 0.609 (SD 0.166) U/g Hb before thiamine therapy and rose to 0.772 (SD 0.152) U/g Hb immediately after the administration of thiamine to the patients. The difference between these values, 0.163 (SD 0.130) U/g, is the mean activity of transketolase protein which can be activated by thiamine in vivo but not by thiamine diphosphate in vitro. This difference correlated with low initial erythrocyte transketolase activity in these patients, but not with their alcohol intake, liver function or diagnoses.