Determination of multiple mycotoxins in paired plasma and urine samples to assess human exposure in Nanjing, China
Determination of multiple mycotoxins in paired plasma and urine samples to assess human exposure in Nanjing, China
复制标题
测定配对血浆和尿液样本中的多种霉菌毒素，以评估中国南京的人体暴露情况
DOI：
10.1016/j.envpol.2019.02.091
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发表时间：
2019-05-01
影响因子：
8.9
通讯作者：
Han, Zheng
中科院分区：
文献类型：
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作者：
Fan, Kai;Xu, Juanjuan;Han, Zheng
本研究调查了南京市260名农村居民（年龄18-66岁）的霉菌毒素暴露情况。通过超高效液相色谱串联质谱（UHPLC-MS/MS）方法分析了配对血浆和第一次晨尿样本中的26种真菌毒素生物标志物，包括12种母体真菌毒素和14种真菌毒素代谢物。在95/260（36.5%）份血浆样本和144/260（55.4%）份尿液样本中检出真菌毒素及其代谢产物。血浆中最常见的霉菌毒素为赭曲霉毒素A（OTA），占27.7%（范围0.312 - 9.18 μ g/L），而黄曲霉毒素B-1-赖氨酸（AF B（1）-赖氨酸）（发生率19.6%，范围10.5-74.5 pg/mg白蛋白），伏马菌素B-1（FB 1）（发病率2.7%，范围0.305-0.993 μ g/L），脱氧雪腐镰刀菌烯醇（DON）（发病率2.3%，范围1.39-5.53微克/升），玉米赤霉烯酮（ZEN）在血浆样品中还检测到玉米赤霉酮（ZAN）（发病率6.5%，范围0.063-0.418 μ g/L）和玉米赤霉酮（ZAN）（发病率1.2%，范围0.164- 0.346 μ g/L）。脱氧雪腐镰刀菌烯醇-15-葡萄糖醛酸苷（DON-15-GlcA）是最常见的尿真菌毒素，发生率为43.8%（范围0.828-37.7 μ g/L）。唐（发生率10.0%，范围1.39-14.7 μ g/L），DON-3-葡糖苷酸（DON-3-GlcA）（发生率15.8%，范围0.583-5.84 μ g/L），黄曲霉毒素M-1（AFM（1））（发病率10.4%，范围0.125-0.464 μ g/L），ZAN（发生率7.7%，范围0.106-1.82 μ g/L），ZEN（发病率6.9%，范围0.056 - 0.311 μ g/L），FB 1（发病率3.1%，范围0.230-1.33 μ g/L），T-2毒素尿中检出OTA（检出率为1.2%，范围为0.153-0.557 μ g/L）。根据血浆或尿液中AFB（1）、FB 1、ZEN、DON和OTA的水平，估算每日摄入量。结果表明，调查的农村居民暴露于多种霉菌毒素，特别是致癌霉菌毒素AFB（1），平均每日摄入量为0.41 μ g/kg.bw/day，从而强调了潜在的公共卫生问题。据我们所知，这是第一项通过直接测量单个人群中200多名受试者的配对血浆和尿液样本中的多种真菌毒素来评估人体暴露于真菌毒素的研究。(C)2019爱思唯尔有限公司版权所有。
This study was conducted to investigate mycotoxin exposure in 260 rural residents (age 18-66 years) in Nanjing, China. Paired plasma and first morning urine samples were analyzed for 26 mycotoxin biomarkers, including 12 parent mycotoxins and 14 mycotoxin metabolites, by an ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method. Mycotoxins and their metabolites were detected in 95/260 (36.5%) plasma samples and 144/260 (55.4%) urine samples. The most prevalent mycotoxin in plasma was ochratoxin A (OTA), with the incidence of 27.7% (range 0.312 -9.18 mu g/L), while aflatoxin B-1-lysine (AFB(1)-lysine) (incidence 19.6%, range 10.5-74.5 pg/mg albumin), fumonisin B-1 (FB1) (incidence 2.7%, range 0.305-0.993 mu g/L), deoxynivalenol (DON) (incidence 2.3%, range 1.39-5.53 mu g/L), zearalenone (ZEN) (incidence 6.5%, range 0.063-0.418 mu g/L) and zearalanone (ZAN) (incidence 1.2%, range 0.164-0346 mu g/L) were also detected in plasma samples. Deoxynivalenol-15-glucuronide (DON-15-GlcA) was the most frequently detected urinary mycotoxin, with the incidence of 43.8% (range 0.828-37.7 mu g/L). DON (incidence 10.0%, range 1.39-14.7 mu g/L), DON-3-glucuronide (DON-3-GlcA) (incidence 15.8%, range 0.583-5.84 mu g/L), aflatoxin M-1 (AFM(1)) (incidence 10.4%, range 0.125-0.464 mu g/L), ZAN (incidence 7.7%, range 0.106-1.82 mu g/L), ZEN (incidence 6.9%, range 0.056 -0.311 mu g/L), FB1 (incidence 3.1%, range 0.230-1.33 mu g/L), T-2 toxin (incidence 2.3%, range 0.248-3.61 mu g/L) and OTA (incidence 1.2%, range 0.153-0.557 mu g/L) were also found in urine samples. Based on the plasma or urinary levels, the daily intakes of AFB(1), FB1, ZEN, DON and OTA were estimated. The results showed that the investigated rural dwellers were exposed to multiple mycotoxins, especially to carcinogenic mycotoxin AFB(1) with a mean daily intake of 0.41 mu g/kg.bw/day, thereby underlining a potential public health concern. To the best of our knowledge, this is the first study to evaluate human exposure to mycotoxins with direct measurements of multiple mycotoxins in paired plasma and urine samples for over 200 subjects of a single population. (C) 2019 Elsevier Ltd. All rights reserved.