Expression of p53 protein in liver and sera of patients with liver fibrosis, liver cirrhosis or hepatocellular carcinoma associated with chronic HCV infection
Expression of p53 protein in liver and sera of patients with liver fibrosis, liver cirrhosis or hepatocellular carcinoma associated with chronic HCV infection
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DOI：
10.1016/j.clinbiochem.2008.11.004
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发表时间：
2009-04-01
影响因子：
2.8
通讯作者：
El-Dosoky, Ibrahim
中科院分区：
文献类型：
--
作者：
Attallah, Abdelfattah M.;Shiha, Gamal E.;El-Dosoky, Ibrahim
目的：丙型肝炎病毒（HCV）是慢性肝炎的主要病原体，它导致肝硬化和肝细胞癌（HCC）的发展。为探讨HCV基因IV型感染者不同肝组织病理类型中p53蛋白和抗p53抗体水平的意义，采用免疫组化和westernblot法检测肝组织和血清中p53蛋白。结果：肝纤维化组（F1 ~ F3）和肝硬化组（F4）肝组织中p53蛋白表达较弱，呈弥漫性，而肝癌组p53蛋白表达较强，呈弥漫性。在肝纤维化、肝硬化和HCC患者的血清中，在53-kDa处鉴定了靶p53蛋白。血清p53蛋白的检出率明显增高（P
Objectives: Hepatitis C virus (HCV) is a major aetiological agent of chronic hepatitis and it leads to the development of liver cirrhosis and hepatocellular carcinoma (HCC). The significances of p53 protein and anti-p53 antibodies levels in HCV genotype IV infected patients with different liver pathology were evaluated.Design and methods: Immunostaining and western blot based oil monospecific anti-p53 antibody Were Used for the identification of p53 protein in liver tissues and serum samples. The serum levels of p53 protein and anti-p53 IgG antibodies were evaluated using enzyme linked immunosorbent assay (ELISA).Results: Mild and diffuse p53 cytoplasmic immunostaining was found in liver tissues of patients with liver fibrosis [F1-F3] and liver cirrhosis [F4] in comparison with strong and diffuse p53 cytoplasmic immunostaining in patients with HCC. The target p53 protein was identified in sera of patients with liver fibrosis, liver cirrhosis and HCC at 53-kDa. The detection rate of serum p53 protein increases significantly (p