A COPPER BLOCK METHOD FOR FREEZING NONCRYOPROTECTED TISSUE TO PRODUCE ICE CRYSTAL-FREE REGIONS FOR ELECTRON MICROSCOPY PART 1 EVALUATION USING FREEZE SUBSTITUTION
A COPPER BLOCK METHOD FOR FREEZING NONCRYOPROTECTED TISSUE TO PRODUCE ICE CRYSTAL-FREE REGIONS FOR ELECTRON MICROSCOPY PART 1 EVALUATION USING FREEZE SUBSTITUTION
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DOI：
10.1111/j.1365-2818.1976.tb02405.x
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发表时间：
1976-01-01
期刊：
影响因子：
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通讯作者：
BULLIVANT S
中科院分区：
文献类型：
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作者：
DEMPSEY G P;BULLIVANT S
各种植物和动物组织制备EM的冷冻置换后，在液体N冷却铜块的货车Harreveld方法。测量未用任何冷冻保护剂处理的组织中的冰晶尺寸与深度。冰晶的大小随着深度的增加而成倍增加。足够快地冷冻窄的表面带（约12 μ m）以防止形成电子显微镜可见的冰晶。
A wide variety of plant and animal tissues were prepared for EM by freeze-substitution, after rapid freezing on a liquid N cooled Cu block by the van Harreveld method. Measurements were made of ice crystal size vs. depth in tissues that had not been treated with any cryoprotectants. Ice crystal size increased exponentially with depth. A narrow surface band (approximately 12 .mu.m) was frozen sufficiently rapidly to prevent the formation of electron microscopically-visible ice crystals.