Prevalence and abundance of latently transcribed varicella-zoster virus genes in human ganglia
Prevalence and abundance of latently transcribed varicella-zoster virus genes in human ganglia
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DOI：
10.1128/jvi.02745-06
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发表时间：
2007-03-01
影响因子：
5.4
通讯作者：
Gilden, Donald H.
中科院分区：
文献类型：
--
作者：
Cohrs, Randall J.;Gilden, Donald H.
在潜伏感染水痘-带状疱疹病毒（VZV）的人神经节中，序列分析揭示了五个病毒基因（VZV基因21、29、62、63和66）被转录。然而，它们的相对流行率和丰度尚不清楚。在这里，使用实时PCR，我们分析了28三叉神经节从14人的RNA对应的五个病毒基因被转录在潜伏感染的人类神经节。发现的最普遍的转录物是VZV基因63（78%），其次是基因66（43%）、基因62（36%）和基因29（21%）。在28个神经节中没有检测到基因21的转录本。VZV基因63 RNA也是在潜伏感染的人神经节中检测到的最丰富的（3，710 +/-6，895拷贝/1 μ g mRNA）转录物，其次是VZV基因29（491 +/-594）、VZV基因66（117 +/-85）和VZV基因62（64 +/-38）。因此，在潜伏感染的神经节中重复检测和高丰度的VZV基因63转录物表明，VZV基因63对于维持病毒潜伏期可能比不太丰富转录和随机检测的VZV基因21、29、62和66更重要。
In human ganglia latently infected with varicella-zoster virus (VZV), sequence analysis has revealed that five viral genes (VZV genes 21, 29, 62, 63, and 66) are transcribed. However, their comparative prevalence and abundance are unknown. Here, using real-time PCR, we analyzed 28 trigeminal ganglia from 14 humans for RNA corresponding to the five virus genes known to be transcribed in latently infected human ganglia. The most prevalent transcript found was VZV gene 63 (78%), followed by gene 66 (43%), gene 62 (36%), and gene 29 (21%). No gene 21 transcripts were detected in any of the 28 ganglia. VZV gene 63 RNA was also the most abundant (3,710 +/- 6,895 copies per I mu g of mRNA) transcript detected in latently infected human ganglia, followed by VZV gene 29 (491 +/- 594), VZV gene 66 (117 +/- 85), and VZV gene 62 (64 +/- 38). Thus, the repeated detection and high abundance of VZV gene 63 transcripts in latently infected ganglia suggests that VZV gene 63 may be more important for the maintenance of virus latency than the less abundantly transcribed and randomly detected VZV genes 21, 29, 62, and 66.