Disaccharide analysis of chondroitin and heparin from farmed Atlantic salmon
Disaccharide analysis of chondroitin and heparin from farmed Atlantic salmon
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DOI：
10.1007/s10719-016-9652-8
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发表时间：
2016-04-01
影响因子：
3
通讯作者：
Flengsrud, Ragnar
中科院分区：
文献类型：
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作者：
Flengsrud, Ragnar
据报道，在养殖的大西洋鲑鱼 (Salmo salar) 鳃和肠道中检测到的肝素二糖（除一例外）在猪肝素中也有报道。两个物种中二糖的相对量似乎有很大不同。在养殖的大西洋鲑鱼中检测到两种软骨素二糖，这两种软骨素二糖在斑马鱼（Danio rerio）的发育和分化中具有重要作用。此外，这里检测到的大多数软骨素/硫酸皮肤素和肝素二糖已在斑马鱼中报道，支持了鱼类中存在肝素的说法。在硬骨鱼的骨头中也检测到了相同的软骨素/皮肤素二糖。在所有 5 种硬骨鱼中都发现了微量的稀有二糖 UA2S-GalNAc，在鳃中的含量相对较高，在肠道中的含量也很高。稀有的肝素二糖 UA2S-GlcN 在鳃和肠中的含量相对最高。结合我们之前的报告，本次通讯表明养殖大西洋鲑鱼肝素中的糖胺聚糖需要进一步研究，以澄清结构和功能。
The heparin disaccharides detected in farmed Atlantic salmon (Salmo salar) gills and intestines have, with one exception, been reported in porcine heparin. The relative amounts of disaccharides appear to be very different in the two species. Two chondroitin disaccharides with a proposed essential role in the zebrafish (Danio rerio) development and differentiation are detected in farmed Atlantic salmon. In addition, most of the chondroitin/dermatan sulfate and heparin disaccharides detected here have been reported in zebrafish, in support of the claims of the heparin presence in fish. The same chondroitin/dermatan disaccharides were detected in the bones of bony fishes. The rare disaccharide UA2S-GalNAc that was found in trace amounts in all 5 bony fishes was found in relative high amounts in gills and in significant amounts in intestines. The rare heparin disaccharide UA2S-GlcN was in relative highest amounts both in gills and intestines. In context with our previous reports, this communication suggests that glycosaminoglycans in farmed Atlantic salmon heparin need further studies in order to clarify structure and function.