Minimal sulfur requirement for growth and sulfur-dependent metabolism of the hyperthermophilic archaeon Staphylothermus marinus.
Minimal sulfur requirement for growth and sulfur-dependent metabolism of the hyperthermophilic archaeon Staphylothermus marinus.
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DOI：
10.1155/2003/626017
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发表时间：
2003-10-01
期刊：
影响因子：
2.4
通讯作者：
Ma, Kesen
中科院分区：
文献类型：
--
作者：
Hao, Xiaolei;Ma, Kesen
海洋葡萄球菌（Staphylothermus marinus）是一种厌氧超嗜热古菌，它利用肽作为碳源和能源。元素硫（Sdegree）是其生长所必需的，并被还原为H2S。通过检测该菌的生长和关键酶的活性，探讨了Sdegree还原的代谢功能和机制。所有三种形式的Sdegree测试-升华Sdegree，胶体Sdegree和多硫化物-被使用的S。marinus，没有其他含硫化合物可以取代Sdegree。元素硫不作为物理支持，但似乎作为电子受体的功能。最佳生长所需的最低Sdegree浓度为0.05%（w/v）。在该浓度下，似乎存在从H2产生到Sdegree还原的代谢转变。在无细胞提取物中检测到硫还原酶、氢酶、谷氨酸脱氢酶和电子传递酶等与S度依赖性代谢有关的酶活性。marinus。这些结果表明Sdegree在S. marinus。由肽解的氨基酸氧化产生的还原当量可以转移到硫还原酶和氢化酶，其然后分别催化H2S和H2的产生。
Staphylothermus marinus is an anaerobic hyperthermophilic archaeon that uses peptides as carbon and energy sources. Elemental sulfur (Sdegree) is obligately required for its growth and is reduced to H2S. The metabolic functions and mechanisms of Sdegree reduction were explored by examining Sdegree-dependent growth and activities of key enzymes present in this organism. All three forms of Sdegree tested-sublimed Sdegree, colloidal Sdegree and polysulfide-were used by S. marinus, and no other sulfur-containing compounds could replace Sdegree. Elemental sulfur did not serve as physical support but appeared to function as an electron acceptor. The minimal Sdegree concentration required for optimal growth was 0.05% (w/v). At this concentration, there appeared to be a metabolic transition from H2 production to Sdegree reduction. Some enzymatic activities related to Sdegree-dependent metabolism, including sulfur reductase, hydrogenase, glutamate dehydrogenase and electron transfer activities, were detected in cell-free extracts of S. marinus. These results indicate that Sdegree plays an essential role in the heterotrophic metabolism of S. marinus. Reducing equivalents generated by the oxidation of amino acids from peptidolysis may be transferred to sulfur reductase and hydrogenase, which then catalyze the production of H2S and H2, respectively.