Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components
Micropatterned cell co-cultures using layer-by-layer deposition of extracellular matrix components
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DOI：
10.1016/j.biomaterials.2005.09.015
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Langer, R
中科院分区：
文献类型：
--
作者：
Fukuda, J;Khademhosseini, A;Langer, R
使用三种主要的细胞外基质组分：透明质酸（HA）、纤连蛋白（FN）和胶原蛋白制造微图案化细胞共培养物。为了制造与这些组分的共培养物，通过毛细管力光刻在玻璃基底上微图案化HA，并且用FN涂覆暴露的玻璃区域以产生细胞粘附岛。一旦第一种细胞类型被固定在粘附岛上。胶原蛋白随后静电吸附到HA图案上，将非粘附的HA表面转变为粘附的，从而促进第二种细胞类型的粘附。该技术利用天然细胞外基质组分，因此提供高生物亲和力且无细胞毒性。这种生物相容性共培养系统可能为研究细胞行为（如细胞-细胞通讯和细胞-基质相互作用）以及组织工程应用提供新的工具。(c)2005爱思唯尔有限公司保留所有权利。
Micropatterned cellular co-cultures were fabricated using three major extracellular matrix components: hyaluronic acid (HA), fibronectin (FN) and collagen. To fabricate co-cultures with these components, HA was micropatterned on a glass substrate by capillary force lithography, and the regions of exposed glass were coated with FN to generate cell adhesive islands. Once the first cell type was immobilized on the adhesive islands. the subsequent electrostatic adsorption of collagen to HA patterns switched the non-adherent HA surfaces to adherent, thereby facilitating the adhesion of a second cell type. This technique utilized native extracellular matrix components and therefore affords high biological affinity and no cytotoxicity. This biocompatible co-culture system could potentially provide a new tool to study cell behavior such as cell-cell communication and cell-matrix interactions, as well as tissue-engineering applications. (c) 2005 Elsevier Ltd. All rights reserved.