TRANSIENT INDUCTION OF PHENYLALANINE AMMONIA-LYASE AND 4-COUMARATE-COA LIGASE MESSENGER-RNAS IN POTATO LEAVES INFECTED WITH VIRULENT OR AVIRULENT RACES OF PHYTOPHTHORA-INFESTANS
TRANSIENT INDUCTION OF PHENYLALANINE AMMONIA-LYASE AND 4-COUMARATE-COA LIGASE MESSENGER-RNAS IN POTATO LEAVES INFECTED WITH VIRULENT OR AVIRULENT RACES OF PHYTOPHTHORA-INFESTANS
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DOI：
10.1104/pp.85.1.34
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发表时间：
1987-09-01
期刊：
影响因子：
7.4
通讯作者：
HAHLBROCK, K
中科院分区：
文献类型：
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作者：
FRITZEMEIER, KH;CRETIN, C;HAHLBROCK, K
马铃薯叶片感染致病疫霉 (Pi) 会导致苯丙素代谢的快速刺激。体外合成的蛋白质的二维凝胶电泳证明，在接种后几小时内即可检测到多种 mRNA 活性的增加，包括编码苯丙氨酸解氨酶 (PAL) 和 4-香豆酸:CoA 连接酶 (4CL) 的 mRNA。通过切下的叶茎施用Pi培养滤液可以密切模拟这种效应，通过Pi培养滤液或花生四烯酸处理，马铃薯细胞悬浮培养物中的PAL和4CL mRNA活性也被快速且短暂地诱导。利用这种诱导来生成与 PAL 和 4CL mRNA 互补的 cDNA 探针。使用这些探针进行的印迹杂交显示，在用 pI 培养物滤液处理的叶子中，转录率几乎是立即的、瞬时的和协调的增加，以及 PAL 和 4CL mRNA 量的后续变化。在携带抗性基因 R1（cv Datura）或 R4（cv Isola）的马铃薯品种的受感染叶子中发现了类似的 mRNA 量变化，与接种时使用的是强毒力还是无毒力 Pi 病型无关。这些结果与最近对相同马铃薯品种和 Pi 致病型的细胞学观察进行了讨论。
Infection of potato leaves with the fungal pathogen Phytophthora infestans (Pi) resulted in the rapid stimulation of phenylpropanoid metabolism. Increases in the activities of several mRNAs, including those encoding phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL), were detectable within a few hours postinoculation, as demonstrated by two-dimensional gel electrophoresis of proteins synthesized in vitro. This effect was closely mimicked by application of Pi culture filtrate through cut leaf stems, PAL and 4CL mRNA activities were also rapidly and transiently induced in potato cell suspension cultures by treatments with Pi culture filtrate or arachidonic acid. This induction was exploited to generate cDNA probes complementary to PAL and 4CL mRNAs. Blot hybridizations using these probes revealed almost immediate, transient and coordinate increases in the transcription rates and subsequent changes in the amounts of PAL and 4CL mRNAs in leaves treated with pI culture filtrate. Similar changes in the mRNA amounts were found in infected leaves of potato cultivars carrying resistance genes R1 (cv Datura) or R4 (cv Isola), independent of whether a virulent or an avirulent Pi pathotype was used for inoculation. These results are discussed in relation to recent cytological observations with the same potato cultivars and Pi pathotypes.