An Intrinsic Distinction in Neuromuscular Junction Assembly and Maintenance in Different Skeletal Muscles
An Intrinsic Distinction in Neuromuscular Junction Assembly and Maintenance in Different Skeletal Muscles
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DOI：
10.1016/s0896-6273(02)00670-0
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发表时间：
2002-04
期刊：
影响因子：
16.2
通讯作者：
S. Pun;M. Sigrist;A. F. Santos;M. Ruegg;J. Sanes;T. Jessell;S. Arber;P. Caroni
中科院分区：
文献类型：
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作者：
S. Pun;M. Sigrist;A. F. Santos;M. Ruegg;J. Sanes;T. Jessell;S. Arber;P. Caroni
我们分析了小鼠胚胎各个肌肉中神经肌肉接头（NMJ）的形成。骨骼肌可分为两类不同的肌肉之一，称为“快速突触”(FaSyn) 和“延迟突触”(DeSyn) 肌肉，它们在突触后 AChR 的初始焦点聚集、突触前成熟的时间以及年轻成年小鼠中 NMJ 的维持方面存在显着差异。类别之间的差异是肌肉固有的，并表现为缺乏神经支配或集聚力。年轻成人肌肉的麻痹或去神经导致 DeSyn 肌肉上的 AChR 簇解体，而 FaSyn 肌肉上的 AChR 簇则被保留。我们的结果表明，FaSyn 和 DeSyn 肌肉固有的突触后分化过程影响 NMJ 在发育过程中的形成及其在成人中的维持。
We analyzed the formation of neuromuscular junctions (NMJs) in individual muscles of the mouse embryo. Skeletal muscles can be assigned to one of two distinct classes of muscles, termed" Fast Synapsing"(FaSyn) and" Delayed Synapsing"(DeSyn) muscles, which differ significantly with respect to the initial focal clustering of postsynaptic AChRs, the timing of presynaptic maturation, and the maintenance of NMJs in young adult mice. Differences between classes were intrinsic to the muscles and manifested in the absence of innervation or agrin. Paralysis or denervation of young adult muscles resulted in disassembly of AChR clusters on DeSyn muscles, whereas those on FaSyn muscles were preserved. Our results show that postsynaptic differentiation processes intrinsic to FaSyn and DeSyn muscles influence the formation of NMJs during development and their maintenance in the adult.