Hepatitis B virus genotype A: design of reference sequences for sub-genotypes
Hepatitis B virus genotype A: design of reference sequences for sub-genotypes
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乙型肝炎病毒A基因型：亚基因型参考序列的设计
DOI：
10.1007/s11262-016-1307-0
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发表时间：
2016-06-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Zhenhua
中科院分区：
文献类型：
--
作者：
Cai, Qun;Zhu, Huilan;Zhang, Zhenhua
乙型肝炎病毒（HBV/A）的基因型A广泛存在，目前分为六个亚基因型。不同亚基因型合适的参考序列可促进HBV/A的研究。然而，目前该病毒的参考序列不足。在目前的工作中，我们从GenBank数据库中检索了442个全长HBV/A基因组序列，并通过系统发育分析将它们分类为亚基因型。利用MEGA6.0软件，采用极大似然法建立不同HBV/A亚基因型的参考序列。我们的分析表明，这些参考序列在系统发育上与已知菌株聚类，表明我们建立的参考序列确实属于正确的亚基因型。HBV/A亚型序列按地理来源选择，分为a1 -南非、a2 -欧洲、a3 -喀麦隆和a5 -海地亚基因型。构建A1、A2、A3、A5亚基因型参考序列，存入GenBank （KP234050-KP234053）。通过系统发育分析，我们进一步确定了HBV/A谱系最近的共同祖先的时间。总之，这些新建立的参考序列可为HBV基因型A的分子生物学和病毒学研究提供合适的参考标准。
Genotype A of hepatitis B virus (HBV/A) is widespread and is currently divided into six sub-genotypes. Suitable reference sequences for different sub-genotypes can facilitate research on HBV/A. However, the current reference sequences for this virus are insufficient. In the present work, we retrieved 442 full-length HBV/A genomic sequences from the GenBank database and classified them into sub-genotypes by phylogenetic analysis. By the maximum likelihood method using the MEGA6.0 software, we established the reference sequences for different HBV/A sub-genotypes. Our analyses demonstrated that these reference sequences clustered phylogenetically with known strains, indicating that the reference sequences we established indeed belonged to the right sub-genotypes. HBV/A subtype sequences were selected by geographic origins and grouped as sub-genotypes including A1-South Africa, A2-Europe, A3-Cameroon, and A5-Haiti. Reference sequences of sub-genotypes A1, A2, A3, and A5 were constructed and deposited into GenBank (KP234050–KP234053). By applying phylogenetic analyses, we further determined the time to most recent common ancestor of HBV/A lineages. In conclusion, these newly established reference sequences can provide suitable reference standards for studies on the molecular biology and virology of HBV genotype A.