A thermophilic apoglucose dehydrogenase as nonconsuming glucose sensor
A thermophilic apoglucose dehydrogenase as nonconsuming glucose sensor
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DOI：
10.1006/bbrc.2000.3172
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发表时间：
2000-08-11
影响因子：
3.1
通讯作者：
Lakowicz, JR
中科院分区：
文献类型：
--
作者：
D'Auria, S;Di Cesare, N;Lakowicz, JR
血糖是糖尿病保健的临床重要分析物。在这份初步报告中，我们描述了一种基于耐热葡萄糖脱氢酶的D-葡萄糖蛋白生物传感器。用8-苯胺基-1-萘磺酸（ANS）非共价标记葡萄糖脱氢酶。ANS标记的酶显示结合葡萄糖后发射强度降低约25%。我们的研究结果表明，使用葡萄糖作为其底物的酶可以在不存在所需辅因子的情况下用作可逆的和非消耗性的葡萄糖传感器。此外，使用非活性脱辅基酶作为可逆传感器的可能性大大扩展了可用作传感器的蛋白质的范围，不仅用于葡萄糖，但对于各种生物化学相关的分析物。(C)北京大学出版社.
Blood glucose is a clinically important analytes for diabetic health care. In this preliminary report we describe a protein biosensor for D-glucose based on a thermostable glucose dehydrogenase. The glucose dehydrogenase was noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS). The ANS-labeled enzyme displayed an approximate 25% decrease in emission intensity upon binding glucose. This decrease can be used to measure the glucose concentration, Our results suggest that enzymes which use glucose as their substrate can be used as reversible and nonconsuming glucose sensors in the absence of required cofactors, Moreover, the possibility of using inactive apoenzymes for a reversible sensor greatly expands the range of proteins which can be used as sensors, not only for glucose, but for a wide variety of biochemically relevant analytes. (C) 2000 Academic Press.