Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method
Optimized Biotransformation of Icariin into Icariside II by β-Glucosidase from Trichoderma viride Using Central Composite Design Method
复制标题
使用中心复合设计方法，通过绿色木霉的 β-葡萄糖苷酶将淫羊藿苷生物转化为淫羊藿苷 II。
DOI：
10.1155/2016/5936947
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发表时间：
2016-01-01
影响因子：
--
通讯作者：
Ding, Yue
中科院分区：
文献类型：
--
作者：
Cheng, Tao;Yang, Jun;Ding, Yue
A crude beta-glucosidase has been produced from Trichoderma viride and used to explore a simple method to prepare icariside II from icariin. The crude enzyme has been studied by zymography method and used for hydrolysis of ICA. To achieve a high conversion rate of ICA, various factors have been studied including pH, reaction time, temperature, initial concentration of enzyme, and initial concentration of ICA through central composite design experiments. In the condition of the optimum hydrolysis parameters with pH 4.0, 41 degrees C, 1.0 mg/mL ICA, and 9.8 U/mL crude beta-glucosidase, the conversion rate of ICA reached 95.03% at 1 h. Moreover, the cytotoxicity test showed that ICA II performed inhibition effects on proliferation of A549 cell, while ICA has no cytotoxicity. It indicated that the hydrolysis transformation study of ICA is valuable for exploration of active new drugs.
