A new model to study repair of gastric mucosa using primary cultured rabbit gastric epithelial cells.
A new model to study repair of gastric mucosa using primary cultured rabbit gastric epithelial cells.
复制标题
利用原代培养的兔胃上皮细胞研究胃粘膜修复的新模型。
DOI：
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发表时间：
1995
影响因子：
2.9
通讯作者：
N. Sato
中科院分区：
文献类型：
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作者：
S. Watanabe;M. Hirose;Xian;Kouhei Maehiro;T. Murai;Osamu Kobayashi;H. Mikami;K. Otaka;A. Miyazaki;N. Sato
采用原代培养的兔胃粘膜细胞，观察创伤修复过程。将主要由粘液细胞组成的汇合的单层胃粘膜细胞片层损伤以形成恒定大小的无细胞区域。通过图像分析定量分析无细胞区的变化。对照组的伤口在36-48 h内恢复;向培养基中加入胎牛血清（FCS）和肝细胞生长因子（HGF）可加速伤口修复，细胞骨架蛋白抑制剂可延缓伤口修复。在正常创面修复过程中，5-溴脱氧尿苷（BrdU）阳性细胞在24-36 h出现于创面周围，完全修复后消失。在FCS和HGF治疗组中，BrdU阳性细胞主要在创伤后12-24 h检测到。在该模型中，伤口修复分为两个步骤：初始细胞迁移阶段和后期增殖阶段。总之，FCS和一些生长因子通过诱导上皮细胞迁移和增殖来加速伤口修复。细胞骨架系统在胃粘膜的正常修复过程中起着重要的作用。
The process of wound repair was investigated using primary cultured rabbit gastric mucosal cells. A confluent monolayer gastric mucosal cell sheet consisting mainly of mucous cells was wounded to make a cell-free area of constant size. The changes in the cell-free area were analyzed quantitatively by image analysis. The wound recovered in 36-48 h in controls; wound repair was accelerated by the addition of fetal calf serum (FCS) and hepatocyte growth factor (HGF) to the medium and was retarded by inhibitors of cytoskeletal proteins. In the process of normal wound repair, 5-bromodeoxyuridine (BrdU)-positive cells appeared around the wound in 24-36 h but disappeared after complete repair. In the FCS- and HGF-treated group, BrdU-positive cells were mainly detected 12-24 h after wounding. In this model the wound was repaired in two steps: an initial cell migration stage and a later proliferation stage. In conclusion, FCS and some growth factors accelerate wound repair with the induction of both epithelial cell migration and proliferation. The cytoskeletal system plays an important role in normal gastric restoration.