Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats
Effect of Syzygium aromaticum extract on immediate hypersensitivity in rats
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DOI：
10.1016/s0378-8741(97)00143-8
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发表时间：
1998-03-01
影响因子：
5.4
通讯作者：
Shin, TY
中科院分区：
文献类型：
--
作者：
Kim, HM;Lee, EH;Shin, TY
本文研究了香薷（Syzygium aromaticum， L.）水提物的药理作用。稳定。桃金娘科（myrtacae）花芽（SAFE）对即刻过敏。SAFE对化合物48/80诱导的大鼠全身过敏反应有抑制作用（IC50 = 31.25 mg/kg, i.p.）。SAFE还能抑制局部免疫球蛋白E （IgE）介导的被动皮肤过敏反应（IC50 = 17.78 mg/kg，静脉注射；IC50 = 19.81 mg/kg，口服）。当SAFE以25 ~ 1000mg /kg的浓度进行预处理时，血清组胺水平呈剂量依赖性降低。此外，SAFE通过化合物48/80或抗二硝基苯IgE，剂量依赖性地抑制大鼠腹膜肥大细胞（RPMC）的组胺释放。与基底细胞相比，加入SAFE后，RPMC中cAMP水平在10 s内瞬间显著升高约47倍。这些结果表明，在体内和体外，SAFE通过抑制肥大细胞的组胺释放来抑制即时超敏反应。(C) 1998爱思唯尔科学爱尔兰有限公司版权所有。
We investigated the effect of aqueous extract of Syzygium aromaticum (L.) Merr. et Ferry (Myrtaceae) flower bud (SAFE) on immediate hypersensitivity. SAFE inhibited compound 48/80-induced systemic anaphylaxis in rats (IC50 = 31.25 mg/kg, i.p.). SAFE also inhibited local immunoglobulin E (IgE)-mediated passive cutaneous anaphylactic reaction (IC50 = 17.78 mg/kg, i.v.; IC50 = 19.81 mg/kg, p.o.). When SAFE was pretreated at concentrations ranging from 25 to 1000 mg/kg, the serum histamine levels were reduced in a dose-dependent manner. Moreover, SAFE dose-dependently inhibited histamine release from rat peritoneal mast cells (RPMC) by compound 48/80 or anti-dinitrophenyl IgE. When SAFE was added, the level of cAMP in RPMC transiently and significantly increased about 47-fold at 10 s compared with that of basal cells. These results indicate that SAFE inhibits immediate hypersensitivity by inhibition of histamine release from mast cells in vivo and in vitro. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.