Association between miR-199a rs74723057 and MET rs1621 polymorphisms and the risk of hepatocellular carcinoma.
Association between miR-199a rs74723057 and MET rs1621 polymorphisms and the risk of hepatocellular carcinoma.
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miR-199a rs74723057和MET rs1621多态性与肝细胞癌风险的关联
DOI：
10.18632/oncotarget.13033
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发表时间：
2016-11-29
期刊：
影响因子：
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通讯作者：
Yu H
中科院分区：
文献类型：
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作者：
Wang Q;Yu X;Li Q;Qin L;Tan S;Zeng X;Qiu X;Tang B;Jin J;Liao W;Qiu M;Tan L;He G;Li X;He S;Yu H
microRNAs（miRNAs）可以在转录后水平调节基因表达，从而影响癌症风险。本研究的目的是在1032例HCC患者和1060例无癌对照中研究miR-199 a rs74723057和MET rs 1621与HCC风险之间的关系。使用MassARRAY基因分型系统对这两个SNP进行基因分型。计算比值比（OR）和95%置信区间（95%CI）以评估相关性的强度。我们发现，与野生型MET rs 1621的AA基因型相比，变异GG基因型与HCC的风险降低相关（OR = 0.24，95%CI = 0.06-0.96，P = 0.043）。未观察到miR-199 a rs74723057与HCC风险之间的关联。此外，两种SNPs对HCC风险存在交互作用，在携带miR-199 a rs74723057 CG/GG基因型的患者中，携带MET rs 1621 GG基因型的患者与携带MET rs 1621 AG/AA基因型的患者相比，HCC风险降低（OR = 0.15，95% CI = 0.03~0.73，P = 0.018）。我们的研究结果表明，MET rs 1621多态性，单独和与miR-199 a rs74723057相结合，可能会影响肝癌的易感性。需要进一步的大规模关联研究和功能研究来验证我们的发现。
MicroRNAs (miRNAs) can regulate gene expression at post-transcriptional levels, thereby influence cancer risk. The aim of the current study is to investigate association between miR-199a rs74723057 and MET rs1621 and HCC risk in 1032 HCC patients and 1060 cancer-free controls. These two SNPs were genotyped by using the Agena MassARRAY genotyping system. Odds ratio (OR) and 95% confidence interval (95%CI) were calculated to assess the strength of the associations. We found that compared with the wild-type AA genotype of MET rs1621, the variant GG genotype was associated with a decreased risk for HCC (OR = 0.24, 95% CI = 0.06–0.96, P = 0.043). No association between miR-199a rs74723057 and HCC risk was observed. In addition, an interaction effect on HCC risk between the selected two SNPs was found. Among those who carried the CG/GG genotypes of miR-199a rs74723057, those who carried the GG genotype of MET rs1621 had a reduced risk of HCC, when compared with those who carried the AG/AA genotypes of MET rs1621 (OR = 0.15, 95% CI = 0.03~0.73, P for interaction = 0.018). Our results suggest that MET rs1621 polymorphism, alone and combined with miR-199a rs74723057, may influence susceptibility to HCC. Further large-scale association studies and functional studies are needed to validate our findings.