Mapping protein distributions on polytene chromosomes by immunostaining.
Mapping protein distributions on polytene chromosomes by immunostaining.
复制标题
通过免疫染色绘制多线染色体上的蛋白质分布图。
作者：
R. Paro
引言多线染色体的强大的大小和结构允许在非常高的分辨率染色体蛋白质分布的映射。该方案描述了从果蝇幼虫制备多线染色体，用感兴趣的蛋白质对染色体进行免疫染色，并用Giemsa和Hoechst进行复染。
INTRODUCTIONThe formidable size and structure of polytene chromosomes allow mapping of chromosomal protein distributions at very high resolution. This protocol describes the preparation of polytene chromosomes from Drosophila larvae, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst.