Genome-wide identification and characterization of apple long-chain Acyl-CoA synthetases and expression analysis under different stresses
Genome-wide identification and characterization of apple long-chain Acyl-CoA synthetases and expression analysis under different stresses
复制标题
苹果长链酰基辅酶A合成酶的全基因组鉴定、表征及不同胁迫下的表达分析
DOI：
10.1016/j.plaphy.2018.09.004
复制
发表时间：
2018
影响因子：
6.5
通讯作者：
Hao Yu-Jin
中科院分区：
文献类型：
--
作者：
Zhang Chun-Ling;Mao Ke;Zhou Li-Jie;Wang Gui-Luan;Zhang Ya-Li;Li Yuan-Yuan;Hao Yu-Jin
长链酰基辅酶A合成酶（LACS）是酰基激活酶超家族的成员，在脂质合成和储存、脂肪酸分解代谢、矢量酰化以及角质和蜡的合成中具有重要作用。在此，基于苹果×国产参考基因组鉴定了11个苹果MdLACS基因，聚类成六组并定位到十条染色体。多重序列比对和保守基序分析表明AtLACS和MdLACS蛋白的序列高度保守。 MdLACS基因启动子区的顺式元件分析揭示了与胁迫反应性和植物激素相关的各种元件。随后，表达分析表明MdLACS基因在不同组织中响应不同的非生物胁迫具有不同的表达谱。为了进一步研究苹果MdLACS基因的功能，分离了MdLACS1并鉴定了其基本功能，并通过转基因方法测定了MdLACS1响应苹果非生物胁迫抗性的功能。结果表明，MdLACS1增强了苹果愈伤组织对聚乙二醇、盐和脱落酸的耐受性，表明MdLACS1是响应非生物胁迫的重要调节因子。最后，对MdLACS蛋白之间的功能互操作性网络进行了预测和分析，以了解与蜡生物合成相关的蛋白质和基因调控网络之间可能的相互作用以及苹果响应非生物胁迫的调控机制。
Long-chain acyl-CoA synthetases (LACSs) are members of the acyl-activating enzyme superfamily that have important roles in lipid synthesis and storage, fatty acid catabolism, vectorial acylation, and synthesis of cutin and wax. Here, 11 appleMdLACSgenes were identified based on theMalus×domesticareference genome, clustered into six groups and mapped to ten chromosomes. Multiple sequence alignment and conserved motifs analyses showed that the sequences of the AtLACS and MdLACS proteins were highly conserved. A cis-element analysis in the promoter regions of theMdLACSgenes revealed various elements related to stress responsiveness and plant hormones. Subsequently, expression analysis demonstrated that theMdLACSgenes had different expression profiles in different tissues in response to various abiotic stresses. To further study the function ofMdLACSgenes in apple,MdLACS1was isolated to identify its basic function, which the function ofMdLACS1in response to apple abiotic stress resistance was determined by the transgenic method. The results showed theMdLACS1enhanced tolerance to polyethylene glycol, salt, and abscisic acid in the apple callus, suggesting thatMdLACS1is an important regulator in response to abiotic stresses. Finally, the functional interoperability network among the MdLACS proteins was predicted and analyzed, which could the understanding of the possible interactions among proteins and genes regulatory networks concerned with wax biosynthesis and regulatory mechanisms in response to abiotic stresses in apple.