Comparative proteomics of Helicobacter species: The discrimination of gastric and enterohepatic Helicobacter species
Comparative proteomics of Helicobacter species: The discrimination of gastric and enterohepatic Helicobacter species
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DOI：
10.1016/j.jprot.2013.07.016
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发表时间：
2014-01-31
影响因子：
3.3
通讯作者：
Cash, Phillip
中科院分区：
文献类型：
--
作者：
Fowsantear, Winita;Argo, Evelyn;Cash, Phillip
幽门螺杆菌是感染胃粘膜的主要人类病原体，并导致包括胃炎和胃癌在内的一系列感染。尽管螺杆菌属内的其他细菌也可以感染胃粘膜，但也有一些螺杆菌种可以感染胃肠道 (GI) 内的其他部位。使用二维凝胶电泳将七种非幽门螺杆菌（须氏螺杆菌、猫螺杆菌、灰螺杆菌、肝螺杆菌、芬氏螺杆菌、胆囊螺杆菌和胆囊螺杆菌）的细胞蛋白质组与更广泛表征的幽门螺杆菌进行比较。不同的螺杆菌物种显示出独特的 2D 蛋白质谱，可以使用 Progenesis SameSpots 软件将它们组合成单个数据集。主成分分析用于寻找细菌蛋白质组与其感染部位之间的相关性。这种方法清楚地区分了胃螺杆菌（即感染胃粘膜的细菌）和肠肝螺杆菌（即感染胃肠道小肠和肝胆区域的细菌）。通过 LC-MS 鉴定了这两组细菌之间丰度存在显着差异的选定蛋白质点。这些数据为定义影响细菌感染部位的细菌蛋白质组特征提供了初步见解。生物学意义这项研究表明，根据使用 2D 凝胶电泳测定的体外全细胞蛋白质组，很容易区分螺杆菌属的代表性成员。尽管观察到种内异质性，但仍有可能证明肠肝螺杆菌（以胆汁螺杆菌、肝螺杆菌、芬氏螺杆菌、灰螺杆菌和胆囊螺杆菌为代表）和胃螺杆菌（以幽门螺杆菌、鼬螺杆菌和猫螺杆菌为代表）根据其 2D 蛋白质谱形成离散的群体。鉴定出与螺杆菌属这些成员的典型定植位点相关的临时蛋白质组特征。 (C) 2013 Elsevier B.V. 保留所有权利。
Helicobacter pylori is a major human pathogen that infects the gastric mucosa and is responsible for a range of infections including gastritis and gastric carcinoma. Although other bacteria within the Helicobacter genus can also infect the gastric mucosa, there are Helicobacter species that infect alternative sites within the gastrointestinal (GI) tract. Two-dimensional gel electrophoresis was used to compare the cellular proteomes of seven non-pylori Helicobacters (H. mustelae, H. felis, H. cinaedi, H. hepaticus, H. fennelliae, H. bilis and H. cholecystus) against the more extensively characterised H. pylori. The different Helicobacter species showed distinctive 2D protein profiles, it was possible to combine them into a single dataset using Progenesis SameSpots software. Principal Component Analysis was used to search for correlations between the bacterial proteomes and their sites of infection. This approach clearly discriminated between gastric (i.e. those which infect in the gastric mucosa) and enterohepatic Helicobacter species (i.e. those bacteria that infect the small intestine and hepatobillary regions of the GI tract). Selected protein spots showing significant differences in abundance between these two groups of bacteria were identified by LC-MS. The data provide an initial insight into defining those features of the bacterial proteome that influence the sites of bacterial infection.Biological significanceThis study demonstrated that representative members of the Helicobacter genus were readily discriminated from each other on the basis of their in vitro whole cell proteomes determined using 2D gel electrophoresis. Despite the intra-species heterogeneity observed it was possible, to demonstrate that the enterohepatic (represented by H. bilis, H. hepaticus, H. fennelliae, H. cinaedi and H. cholecystus) and gastric (represented by H. pylori, H. mustelae, and H. felis) Helicobacters formed discrete groups based on their 2D protein profiles. A provisional proteomic signature was identified that correlated with the typical sites of colonisation of these members of the Helicobacter genus. (C) 2013 Elsevier B.V. All rights reserved.