Subchronic feeding study of high-free-lysine transgenic rice in Sprague-Dawley rats.
Subchronic feeding study of high-free-lysine transgenic rice in Sprague-Dawley rats.
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DOI：
10.1016/j.fct.2017.04.023
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
Qing-qing Yang;Xiaoyun He;Hong-yu Wu;Changquan Zhang;Shiying Zou;Tianqi Lang;S. S. Sun-S.;Qiaoquan Liu
中科院分区：
文献类型：
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作者：
Qing-qing Yang;Xiaoyun He;Hong-yu Wu;Changquan Zhang;Shiying Zou;Tianqi Lang;S. S. Sun-S.;Qiaoquan Liu
赖氨酸被认为是大米中的第一必需氨基酸。高游离赖氨酸转基因优良品系HFL1是通过代谢工程获得的调节赖氨酸代谢的转基因品系。本研究进行了为期90天的毒理学实验，研究了不同剂量的HFL1大米对SD大鼠的潜在健康影响。在试验期间，记录了大鼠的体重增加、食物消耗和食物效率，并且与非转基因(N)或对照饲料相比，转基因(T)大米饲料没有观察到不良影响。在中期和最终评估中，测量了血液学参数和血清化学，并在试验结束时检查了器官重量和组织病理学。饲喂T型饲料的大鼠与饲喂N或对照饲料的大鼠在大多数血液学或血清化学参数或器官重量方面没有与饮食相关的差异。T组与其相应的N组和/或对照组之间的一些参数有所不同，但没有观察到不良的组织学影响。综上所述，目前试验的数据表明，在90天内，高赖氨酸转基因大米在摄入高达70%HFL1大米的饮食中对Spraogue-Dawley大鼠没有不良影响。
Lysine is considered to be the first essential amino acid in rice. An eliteHigh-Free-Lysine transgenic line HFL1 was previously produced by metabolic engineering to regulate lysine metabolism. In this study, a 90-day toxicology experiment was undertaken to investigate the potential health effect of feeding different doses of HFL1 rice to Sprague-Dawley rats. During the trial, body weight gain, food consumption and food efficiency were recorded, and no adverse effect was observed in rats fed transgenic (T) rice diets compared with non-transgenic (N) or control diets. At both midterm and final assessments, hematological parameters and serum chemistry were measured, and organ weights and histopathology were examined at the end of the trial. There was no diet-related difference in most hematological or serum chemistry parameters or organ weights between rats fed the T diets and those fed the N or control diets. Some parameters were found to differ between T groups and their corresponding N and/or control groups, but no adverse histological effect was observed. Taken together, the data from the current trial demonstrates that high lysine transgenic rice led to no adverse effect in Sprague-Dawley rats given a diet containing up to 70% HFL1 rice in 90 days.