Discordant temporal development of bacterial phyla and the emergence of core in the fecal microbiota of young children
Discordant temporal development of bacterial phyla and the emergence of core in the fecal microbiota of young children
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DOI：
10.1038/ismej.2015.177
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发表时间：
2016-04-01
期刊：
影响因子：
11
通讯作者：
Satokari, Reetta
中科院分区：
文献类型：
--
作者：
Cheng, Jing;Ringel-Kulka, Tamar;Satokari, Reetta
儿童时期肠道微生物群的定植模式可能会影响以后的健康，但对 1 岁以上儿童的研究很少。我们对 1-4 岁的健康儿童 (n=28) 进行了长达 12 个月的跟踪，在此期间进行了合生素干预并偶尔摄入抗生素，并将他们与来自同一地区的成年人进行了比较。使用 HITChip 系统发育微阵列对微生物群进行定量，并使用线性混合效应模型和其他统计方法进行分析。合生元施用增加了放线菌的稳定性，而抗生素则降低了梭菌簇 XIVa 的丰度。 1至5岁儿童的细菌多样性并没有增加，仍然显着低于成人。放线菌、杆菌和梭状芽胞杆菌 IV 簇保留了儿童般的丰度，而其他一些群体正在转变为成人般的特征。微生物群稳定性增加，其中拟杆菌是主要贡献者。儿童微生物群的共同核心随着年龄的增长而增加，从 18 岁到 25 岁，高度丰富的属级分类单元（包括几种产生丁酸盐的生物体），并向成人样的组成发展。总之，肠道微生物群在 5 岁之前尚未建立，其多样性、核心微生物群和不同类群仍在向成人型配置发展。细菌门的不一致发育模式可能反映了儿童的生理发育步骤。
The colonization pattern of intestinal microbiota during childhood may impact health later in life, but children older than 1 year are poorly studied. We followed healthy children aged 1-4 years (n=28) for up to 12 months, during which a synbiotic intervention and occasional antibiotics intake occurred, and compared them with adults from the same region. Microbiota was quantified with the HITChip phylogenetic microarray and analyzed with linear mixed effects model and other statistical approaches. Synbiotic administration increased the stability of Actinobacteria and antibiotics decreased Clostridium cluster XIVa abundance. Bacterial diversity did not increase in 1- to 5-year-old children and remained significantly lower than in adults. Actinobacteria, Bacilli and Clostridium cluster IV retained child-like abundances, whereas some other groups were converting to adult-like profiles. Microbiota stability increased, with Bacteroidetes being the main contributor. The common core of microbiota in children increased with age from 18 to 25 highly abundant genus-level taxa, including several butyrate-producing organisms, and developed toward an adult-like composition. In conclusion, intestinal microbiota is not established before 5 years of age and diversity, core microbiota and different taxa are still developing toward adult-type configuration. Discordant development patterns of bacterial phyla may reflect physiological development steps in children.