The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats.
The protective effects of chronic intermittent hypobaric hypoxia pretreatment against collagen-induced arthritis in rats.
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DOI：
10.1186/s12950-015-0068-1
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Shi M;Cui F;Liu AJ;Ma HJ;Cheng M;Song SX;Yuan F;Li DP;Zhang Y
探讨慢性间歇性低压缺氧（CIHH）预处理对胶原诱导性关节炎（CIA）大鼠免疫学影响的机制。实验中使用了54只成年雄性Sprague-Dawley大鼠。CIA大鼠（n=18）通过注射胶原诱导关节炎。CIHH+CIA大鼠（n=18）在CIA前给予CIHH（模拟海拔3000 m，每天5 h，持续28 d，PO 2 =108.8 mmHg）。对照组大鼠（n=18）不给予任何处理。(1)CIHH+CIA大鼠CIA发病率明显低于CIA大鼠（P<0.05）。(2)CIHH+CIA大鼠的足爪厚度和关节炎指数（AI）值均低于CIA大鼠（P<0.05）。(3)CIHH+CIA大鼠关节滑膜组织增生伴炎性细胞浸润较CIA大鼠明显减轻。(4)CIHH+CIA大鼠关节滑膜组织和血清中TNF-α、IFN-γ、IL-4和IL-17水平较CIA大鼠明显降低（P<0.05）。(5)CIHH+CIA大鼠外周血CD 4 + T淋巴细胞数及CD 4/CD 8比值低于CIA大鼠（P<0.05）。(6)CIHH+CIA大鼠关节滑膜组织中HIF-1α和NF-κB蛋白表达较CIA大鼠降低（P<0.05）。CIHH预处理通过下调HIF-1α和NF-κB的表达，抑制炎性细胞因子TNF-α和IL-17的表达，平衡CD 4/CD 8和Th 1/Th 2 T淋巴细胞，对胶原诱导的大鼠关节炎具有保护作用。
To explore the immunological mechanisms underlying the effect of chronic intermittent hypobaric hypoxia (CIHH) pretreatment on collagen-induced arthritis (CIA) in rat. Fifty-four adult male Sprague–Dawley rats were used in the experiment. Arthritis in CIA rats (n=18) was induced by injection of collagen. The CIHH+CIA rats (n=18) were treated with CIHH (simulated 3000 m altitude, 5 hours per day for 28 days, PO2=108.8 mmHg) before CIA. The control rats (n=18) were not given any treatment. (1) Incidence rate of CIA in CIHH+CIA rats was significantly lower than that in CIA rats (P<0.05). (2) The paw thickness and arthritis index (AI) value in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (3) The hyperplasia with inflammatory infiltration in synovial tissue of joints in CIHH+CIA rats was much alleviative compared with CIA rats. (4) TNF-α, IFN-γ, IL-4 and IL-17 in synovial tissue of joint and serum in CIHH+CIA rats were decreased compared with CIA rats (P<0.05). (5) The number of CD4-positive T-lymphocytes and the ratio of CD4/CD8 T-lymphocytes in peripheral blood in CIHH+CIA rats were lower than those in CIA rats (P<0.05). (6) The protein expression of HIF-1α and NF-κB in synovial tissue of joint in CIHH+CIA rats was decreased compared with CIA rats (P<0.05). CIHH pretreatment has a protective effect against collagen-induced arthritis in rat through down-regulation of HIF-1α and NF-κB, inhibition of inflammatory cytokines TNF-α and IL-17, and balance in CD4/CD8 and Th1/Th2 T lymphocytes.
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在类风湿滑膜滑膜成纤维细胞激活IL-17和TNFALPHA后，长时间的粒细胞巨噬细胞刺激性刺激因子依赖性，中性粒细胞存活。
影响因子：
4.9
作者：
Parsonage, Greg;Filer, Andrew;Bik, Magdalena;Hardie, Debbie;Lax, Sian;Howlett, Katherine;Church, Leigh D.;Raza, Karim;Wong, See-Heng;Trebilcock, Emily;Scheel-Toellner, Dagmar;Salmon, Mike;Lord, Janet M.;Buckley, Christopher D.
通讯作者：
Buckley, Christopher D.
影响因子：
4.9
作者：
Giatromanolaki, A;Sivridis, E;Koukourakis, MI
通讯作者：
Koukourakis, MI
影响因子：
7.4
作者：
van Patot, Martha C. Tissot;Serkova, Natalie J.;Honigman, Benjamin
通讯作者：
Honigman, Benjamin
DOI：
10.1152/ajpheart.00410.2009
发表时间：
2010-04-01
影响因子：
4.8
作者：
Guan, Yue;Gao, Lu;Zhang, Yi
通讯作者：
Zhang, Yi
影响因子：
4.3
作者：
Deng, Shaohua;Xi, Yebin;Chen, Guangjie
通讯作者：
Chen, Guangjie