DEREPRESSION OF A NOVEL CLASS OF VACCINIA VIRUS GENES UPON DNA-REPLICATION
DEREPRESSION OF A NOVEL CLASS OF VACCINIA VIRUS GENES UPON DNA-REPLICATION
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DOI：
10.1002/j.1460-2075.1988.tb03224.x
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发表时间：
1988-11-01
期刊：
影响因子：
11.4
通讯作者：
STUNNENBERG, HG
中科院分区：
文献类型：
--
作者：
VOS, JC;STUNNENBERG, HG
一种新型的牛痘病毒基因，称为中间基因，在复制后和晚期基因转录开始之前立即表达。中间转录依赖于反式作用因子，在DNA复制开始之前，反式作用因子在病毒感染的细胞中处于活跃状态。质粒携带的中间基因转染到牛痘病毒感染的细胞中，在DNA复制之前表达，而病毒基因组内的拷贝被抑制。DNA复制是激活病毒中间转录的必要条件，复制后不需要重新合成蛋白质。相反，晚期转录的激活依赖于DNA复制和持续的从头合成蛋白质。因此，中间蛋白的一个子集可能是晚期基因转录的反式激活因子。无细胞提取物以类似于在体内观察到的时间表达的方式差异转录早期，中期和晚期基因。讨论了病毒生命周期中基因表达调控的级联模型。
A novel class of vaccinia virus genes, called intermediate, is expressed immediately post-replication and prior to the onset of late gene transcription. Intermediate transcription is dependent on trans-acting factors which are present in an active state in virus-infected cells prior to the onset of DNA replication. Plasmid-borne intermediate genes transfected into vaccinia-virus infected cells are expressed prior to DNA replication, whereas the copies within the viral genome are repressed. DNA replication is essential for activation of viral intermediate transcription and de novo protein synthesis is not required post-replication. In contrast, activation of late transcription depends on DNA replication and continued de novo protein synthesis. Therefore, a subset of intermediate proteins is likely to be trans-activators of late gene transcription. Cell-free extracts differentially transcribe early, intermediate and late genes in a way similar to the temporal expression observed in vivo. A cascade model is discussed for the regulation of gene expression during the viral life-cycle.