Cleavage of RNA phosphodiester bonds by small molecular entities: a mechanistic insight
Cleavage of RNA phosphodiester bonds by small molecular entities: a mechanistic insight
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DOI：
10.1039/c0ob00486c
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Lonnberg, Harri
中科院分区：
文献类型：
--
作者：
Lonnberg, Harri
RNA分子作为遗传信息的载体或催化剂参与许多基本的细胞过程，因此，RNA作为化学治疗剂和化学治疗的靶标受到越来越多的关注。此外，RNA的双重性质导致了RNA世界的概念，即假设生命早期阶段的进化是基于RNA样寡聚体，RNA样寡聚体负责信息的储存和传递，并作为催化剂维持原始代谢。因此，RNA磷酸二酯键裂解的动力学和机制已引起人们的兴趣，并希望它们能阐明酶的作用机制和人工酶的发展。本文综述了近年来关于小分子和离子裂解的主要机理及其对RNA水解人工催化剂的研究进展。
RNA molecules participate in many fundamental cellular processes either as a carrier of genetic information or as a catalyst, and hence, RNA has received increasing interest both as a chemotherapeutic agent and as a target of chemotherapy. In addition the dual nature of RNA has led to the RNA-world concept, i.e. an assumption that the evolution at an early stage of life was based on RNA-like oligomers that were responsible for the storage and transfer of information and as catalysts maintained primitive metabolism. Accordingly, the kinetics and mechanisms of the cleavage of RNA phosphodiester bonds have received interest and it is hoped they will shed light on the mechanisms of enzyme action and on the development of artificial enzymes. The major mechanistic findings concerning the cleavage by small molecules and ions and their significance for the development of efficient and biologically applicable artificial catalysts for RNA hydrolysis are surveyed in the present perspective.