Dynamics of the unbound head during myosin V processive translocation
Dynamics of the unbound head during myosin V processive translocation
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DOI：
10.1038/nsmb1206
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发表时间：
2007-03-01
影响因子：
16.8
通讯作者：
Spudich, James A.
中科院分区：
文献类型：
--
作者：
Dunn, Alexander R.;Spudich, James A.
Myosin V moves cargoes along actin filaments by walking hand over hand. Although numerous studies support the basic hand-over-hand model, little is known about the fleeting intermediate that occurs when the rear head detaches from the filament. Here we use submillisecond dark-field imaging of gold nanoparticle-labeled myosin V to directly observe the free head as it releases from the actin filament, diffuses forward and rebinds. We find that the unbound head rotates freely about the lever-arm junction, a trait that likely facilitates travel through crowded actin meshworks.
